Difference between revisions of "Part:BBa K1065103"
 
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<partinfo>BBa_K1065103 short</partinfo>
 
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This BioBrick contains the coding sequence for the two enzymes pCHA and pCHB. pCHB is the isochorismate-pyruvate lyase while pCHA is the salicylate biosynthesis isochorismate both extracted by the microorganism Pseudomonas aeruginosa. (qua metti i dettagli della reazione o li descrivi solamente nel device completo)
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This BioBrick contains the coding sequence for the two enzymes PchA and PchB.
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This part was submitted in pSB1C3 to the registry by the UniTN-Trento 2013, using an original part of iGEM MIT 2006 <partinfo>BBa_J45319</partinfo> in pSB1AT3.<br>
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This part was cloned by the iGEM UniTN Trento 2013 team for the creation of Wintergreen Devices <partinfo>BBa_K1065102</partinfo> and <partinfo>BBa_K1065106</partinfo>.  
  
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===Usage and Biology===
 
===Usage and Biology===
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PchB is a isochorismate-pyruvate lyase while PchA is a isochorismate synthase, both from the microorganism Pseudomonas aeruginosa. These enzymes are responsible for the production of salicylate acid.
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<center><img style="width:700px;"src="https://static.igem.org/mediawiki/parts/d/df/Tn-2013pchba.jpg"></center>
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<center><p style="width:100px; margin-bottom:60px; text-align:justify">.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1065103 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1065103 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 18:25, 3 October 2013

PchBA

This BioBrick contains the coding sequence for the two enzymes PchA and PchB. This part was submitted in pSB1C3 to the registry by the UniTN-Trento 2013, using an original part of iGEM MIT 2006 BBa_J45319 in pSB1AT3.

This part was cloned by the iGEM UniTN Trento 2013 team for the creation of Wintergreen Devices BBa_K1065102 and BBa_K1065106.

Usage and Biology

PchB is a isochorismate-pyruvate lyase while PchA is a isochorismate synthase, both from the microorganism Pseudomonas aeruginosa. These enzymes are responsible for the production of salicylate acid.

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 326
    Illegal NgoMIV site found at 730
    Illegal NgoMIV site found at 855
    Illegal NgoMIV site found at 866
    Illegal NgoMIV site found at 1147
    Illegal NgoMIV site found at 1615
  • 1000
    COMPATIBLE WITH RFC[1000]