Difference between revisions of "Part:BBa K1065101"
 
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<partinfo>BBa_K1065101 short</partinfo>
 
<partinfo>BBa_K1065101 short</partinfo>
  
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This is the part BBa_K1065100 put under the control of the inducibile promoter AraCpBAD. In this way we were able to obtain the production of Methyl Salicylate only when 5 mM of Arabinose was added to our coltures
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The part <partinfo>BBa_K1065100</partinfo> is under the control of the inducible promoter AraC-pBAD. This promoter allows to produce methyl salicylate starting from salicylic acid when Arabinose is added to the cell culture.
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This part was cloned by the iGEM UniTN Trento 2013 team for the construction of
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Wintergreen Devices <partinfo>BBa_K1065102</partinfo> and <partinfo>BBa_K1065106</partinfo>.
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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BSMT1 catalyzes the conversion of salicylic acid to methyl salicylate, which has a wintergreen smell.
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The arabinose inducible promoter <partinfo>BBa_K731201</partinfo> was fully characterized by iGEM UniTN Trento 2012.
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<html>
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<center><img style="width:500px;"src="https://static.igem.org/mediawiki/parts/6/66/Tn-2013meth.jpg"></center>
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</html>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K1065100 parameters</partinfo>
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Latest revision as of 18:48, 3 October 2013

AraCpBAD + BSMT1

The part BBa_K1065100 is under the control of the inducible promoter AraC-pBAD. This promoter allows to produce methyl salicylate starting from salicylic acid when Arabinose is added to the cell culture.


This part was cloned by the iGEM UniTN Trento 2013 team for the construction of Wintergreen Devices BBa_K1065102 and BBa_K1065106.


Usage and Biology

BSMT1 catalyzes the conversion of salicylic acid to methyl salicylate, which has a wintergreen smell.

The arabinose inducible promoter BBa_K731201 was fully characterized by iGEM UniTN Trento 2012.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1893
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1434
    Illegal SapI site found at 961