Difference between revisions of "Part:BBa K1190000:Design"
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+ | [[File:MelA ov-seq.jpg|MelA ov-seq]] | ||
+ | This part has been sequence confirmed, quantitatively demonstrating correct identity of the biobrick. | ||
===Source=== | ===Source=== |
Latest revision as of 10:20, 5 October 2013
Melan-A (MART-1)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The natural Melan-A sequence has a PstI site at 323bp. This can be remedied by creating a silent mutation to get rid of the PstI site. Because the epitope site is from amino acids 27-35 (81-105bp), it is also possible to shorten the sequence to less than 323 base pairs to exclude the PstI site altogether while retaining protein function as a vaccine candidate.
This part has been sequence confirmed, quantitatively demonstrating correct identity of the biobrick.
Source
Homo sapiens melanocytes