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<h2>Team Bielefeld-CeBiTec 2015</h2>
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We used a modified version of team BIT 2013´s chromium biosensor. We used an <i>E. coli</i> codon optimzed version of the repressor <a href"https://parts.igem.org/Part:BBa_K1758310">BBa_K1758310</a> and combined it with their chromium responsive promoter which is upstrem of a expression enhancing 5´UTR and sfGFP <a href"https://parts.igem.org/Part:BBa_K1758312">BBa_K1758312</a>. Using this two we could characterize the chromium sensor <i>in vitro</i> in our <a href"http://2015.igem.org/Team:Bielefeld-CeBiTec/Project/CFPS">CFPS</a> system.
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Latest revision as of 20:54, 20 September 2015


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Team Bielefeld-CeBiTec 2015

We used a modified version of team BIT 2013´s chromium biosensor. We used an E. coli codon optimzed version of the repressor BBa_K1758310 and combined it with their chromium responsive promoter which is upstrem of a expression enhancing 5´UTR and sfGFP BBa_K1758312. Using this two we could characterize the chromium sensor in vitro in our CFPS system.