Difference between revisions of "Part:BBa K1216003:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1216003 short</partinfo> | <partinfo>BBa_K1216003 short</partinfo> | ||
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No illegal restriction sites. | No illegal restriction sites. | ||
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| + | We obtain the plasmid with the hydrolase from Johannes Haerle of the Panke Group from D-BSSE ETHZ in Basel, checked it for biobrick restriction sites and removed them. We sequenced them to confirm the sequence and did PCR with overlapping fragments to introduce the biobrick pre and suffix to clone them into biobrick vectors. | ||
===Source=== | ===Source=== | ||
Latest revision as of 03:53, 5 October 2013
beta-N-Acetylglucosaminidase (nagZ) from Escherichia Coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 25
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 76
Design Notes
No illegal restriction sites.
We obtain the plasmid with the hydrolase from Johannes Haerle of the Panke Group from D-BSSE ETHZ in Basel, checked it for biobrick restriction sites and removed them. We sequenced them to confirm the sequence and did PCR with overlapping fragments to introduce the biobrick pre and suffix to clone them into biobrick vectors.
Source
E.coli