Difference between revisions of "Assembly:Parts in plasmids"
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BioBrick parts, devices, and systems are distributed, stored, and assembled in plasmids. | BioBrick parts, devices, and systems are distributed, stored, and assembled in plasmids. | ||
| − | An example device consisting of a blue, a green, and a gray part is illustrated to the left on a flattened, circular plasmid. On either side of the three parts, small square red terminators isolate transcription into and out of the BioBrick assembly. The restriction sites for EcoRI, XbaI, SpeI, and PstI are shown as short black vertical lines marked with the initial of the enzyme. Scars left over from earlier stages of assembly are labeled M, which denotes the "mixed" restriction site that is formed by the ligation of SpeI-XbaI sticky ends. (See [[ | + | An example device consisting of a blue, a green, and a gray part is illustrated to the left on a flattened, circular plasmid. On either side of the three parts, small square red terminators isolate transcription into and out of the BioBrick assembly. The restriction sites for EcoRI, XbaI, SpeI, and PstI are shown as short black vertical lines marked with the initial of the enzyme. Scars left over from earlier stages of assembly are labeled M, which denotes the "mixed" restriction site that is formed by the ligation of SpeI-XbaI sticky ends. (See [[Assembly:Restriction enzymes|Restriction Enzymes]] for details.) |
The plasmid also carries a constituitively expressed antibiotic resistance gene indicated by the yellow arrow. | The plasmid also carries a constituitively expressed antibiotic resistance gene indicated by the yellow arrow. | ||
Latest revision as of 23:29, 26 June 2006
BioBrick parts, devices, and systems are distributed, stored, and assembled in plasmids.
An example device consisting of a blue, a green, and a gray part is illustrated to the left on a flattened, circular plasmid. On either side of the three parts, small square red terminators isolate transcription into and out of the BioBrick assembly. The restriction sites for EcoRI, XbaI, SpeI, and PstI are shown as short black vertical lines marked with the initial of the enzyme. Scars left over from earlier stages of assembly are labeled M, which denotes the "mixed" restriction site that is formed by the ligation of SpeI-XbaI sticky ends. (See Restriction Enzymes for details.)
The plasmid also carries a constituitively expressed antibiotic resistance gene indicated by the yellow arrow.
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See also: