Difference between revisions of "Part:BBa K1195007:Design"
m |
|||
| (3 intermediate revisions by one other user not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1195007 short</partinfo> | <partinfo>BBa_K1195007 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | Soeing PCR was used to fuse the YdiV promoter (right up to the initiating ATG) to superfolder GFP. | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | YdiV comes from E. coli. Superfolder GFP is from the iGEM registry. | |
===References=== | ===References=== | ||
| + | Zhou, Xianxuan, Xiaoming Meng, and Baolin Sun. "An EAL domain protein and cyclic AMP contribute to the interaction between the two quorum sensing systems in Escherichia coli." Cell research 18.9 (2008): 937-948. | ||
| + | |||
| + | Wada, Takeo, et al. "EAL domain protein YdiV acts as an anti-FlhD4C2 factor responsible for nutritional control of the flagellar regulon in Salmonella enterica Serovar Typhimurium." Journal of bacteriology 193.7 (2011): 1600-1611. | ||
Latest revision as of 06:22, 28 September 2013
promoter ydiV + GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Soeing PCR was used to fuse the YdiV promoter (right up to the initiating ATG) to superfolder GFP.
Source
YdiV comes from E. coli. Superfolder GFP is from the iGEM registry.
References
Zhou, Xianxuan, Xiaoming Meng, and Baolin Sun. "An EAL domain protein and cyclic AMP contribute to the interaction between the two quorum sensing systems in Escherichia coli." Cell research 18.9 (2008): 937-948.
Wada, Takeo, et al. "EAL domain protein YdiV acts as an anti-FlhD4C2 factor responsible for nutritional control of the flagellar regulon in Salmonella enterica Serovar Typhimurium." Journal of bacteriology 193.7 (2011): 1600-1611.