Difference between revisions of "Part:BBa K1195007"

 
m
 
(One intermediate revision by one other user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1195007 short</partinfo>
 
<partinfo>BBa_K1195007 short</partinfo>
−
 
+
This part is a fusion of the E. coli Ydi V promoter (right up to the ATG encoding YdiV) and superfolder GFP. It will serve as a readout of the transcriptional regulator SdiA activity.
−
The promoter ydiV is know to be turned on when signaled by SdiA. RFP is a common signal protein.  
+
T
−
 
+
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
−
 
+
he E. coli promoter that expressed YdiV is know to be activated by the transcriptional regulator SdiA.  SdiA is a LuxR homolog that senses non-specific quarem-sensing molecules (AHL's).
 +
SdiA has been shown to be activated by a wide variety of AHL's (non-specific AHL's).  We have shown that V. cholera also activates SdiA and created this fusion to detect SdiA activity more easily.
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 06:20, 28 September 2013

promoter ydiV + GFP This part is a fusion of the E. coli Ydi V promoter (right up to the ATG encoding YdiV) and superfolder GFP. It will serve as a readout of the transcriptional regulator SdiA activity. T Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]