Difference between revisions of "Part:BBa K1195003:Design"

 
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<partinfo>BBa_K1195003 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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The hard terminator was added.  
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The promoter Qrr4 from Cholera is known to be active normally, but when Vibrio cholerae senses its own quorum molecules it is turned off. We have attached the promoter Qrr4 to lead into RFP.
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===Source===
 
===Source===
  
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Qrr4 is from Vibrio Cholerae. RFP is from the plasmid used to send parts to iGEM.
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Qrr4 is from Vibrio Cholerae. RFP is from pSB1C3.
  
 
===References===
 
===References===
 +
Svenningsen, Sine L., Kimberly C. Tu, and Bonnie L. Bassler. "Gene dosage compensation calibrates four regulatory RNAs to control Vibrio cholerae quorum sensing." The EMBO journal 28.4 (2009): 429-439.
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Hammer, Brian K., and Sine Lo Svenningsen. "Small RNA target genes and regulatory connections in the Vibrio cholerae quorum sensing system." Quorum Sensing. Humana Press, 2011. 189-206.
 +
 +
Perez, Lark J., et al. "Role of the CAI-1 Fatty Acid Tail in the Vibrio cholerae Quorum Sensing Response." Journal of medicinal chemistry 55.22 (2012): 9669-9681.
 +
 +
Lenz, Derrick H., et al. "The Small RNA Chaperone Hfq and Multiple Small RNAs Control Quorum Sensing in< i> Vibrio harveyi</i> and< i> Vibrio cholerae</i>." Cell 118.1 (2004): 69-82.

Latest revision as of 00:48, 28 September 2013

Promoter Qrr4 + RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 741
    Illegal AgeI site found at 853
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter Qrr4 from Cholera is known to be active normally, but when Vibrio cholerae senses its own quorum molecules it is turned off. We have attached the promoter Qrr4 to lead into RFP.

Source

Qrr4 is from Vibrio Cholerae. RFP is from pSB1C3.

References

Svenningsen, Sine L., Kimberly C. Tu, and Bonnie L. Bassler. "Gene dosage compensation calibrates four regulatory RNAs to control Vibrio cholerae quorum sensing." The EMBO journal 28.4 (2009): 429-439.

Hammer, Brian K., and Sine Lo Svenningsen. "Small RNA target genes and regulatory connections in the Vibrio cholerae quorum sensing system." Quorum Sensing. Humana Press, 2011. 189-206.

Perez, Lark J., et al. "Role of the CAI-1 Fatty Acid Tail in the Vibrio cholerae Quorum Sensing Response." Journal of medicinal chemistry 55.22 (2012): 9669-9681.

Lenz, Derrick H., et al. "The Small RNA Chaperone Hfq and Multiple Small RNAs Control Quorum Sensing in< i> Vibrio harveyi</i> and< i> Vibrio cholerae</i>." Cell 118.1 (2004): 69-82.