Difference between revisions of "Part:BBa K1014001:Design"

Latest revision as of 06:17, 26 September 2013

HrpR Gene

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 534
Illegal AgeI site found at 121
Illegal AgeI site found at 127
Illegal AgeI site found at 175
Illegal AgeI site found at 741
Illegal AgeI site found at 766
1000
COMPATIBLE WITH RFC[1000]

Design Notes

TAATAA double stop codon added.

Codon optimization for E.coli was done using [http://www.jcat.de/ JCat] an online Codon Adaptation Tool which was published in NAR (Nucleic Acids Research).

Codon optimization results was present on [http://2013.igem.org/Team:HIT-Harbin/Parts our wiki]

Source

[http://www.ncbi.nlm.nih.gov/nuccore/NC_007005.1?report=genbank&from=1351297&to=1352241 GeneBank NC_007005.1]

BBa_M39015

References

[http://www.pnas.org/content/102/31/11064.short Proc. Natl. Acad. Sci. U.S.A. 102 (31), 11064-11069 (2005)]

[http://nar.oxfordjournals.org/content/33/suppl_2/W526.short Andreas Grote et al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1014001 short</partinfo>
@@ Line 7: / Line 6: @@
 ===Design Notes===
-ATGTCTACCGACATCGACAACGACGTTCTGACCTGCTGCAACGTTACCGCTCTGTCTGCTGGTCACCAGATCGTTATGAACTCTGTTCTGATGGACATGGACCTGCTGCTGTGCGGTGAAACCGGTACCGGTAAAGACACCCTGGCTTCTCGTATCCACGAACTGTCTTCTCGTACCGGTCCGTTCGTTGGTATGAACTGCGCTGCTATCCCGGAATCTCTGGCTGAATCTCAGCTGTTCGGTGTTGTTAACGGTGCTTTTACTGGTGTCTGCCGTGCTCGTGAAGGTTACATCGAAGCTTCTTCTGGTGGTACCCTGTACCTGGACGAAATCGACTCTATGCCGCTGTCTCTGCAAGCTAAACTGCTGCGTGTTCTGGAATCTCGTGGTGTTGAACGTCTGGGTTCTACCGACTTCATCCCGCTGGACCTGCGTGTTATCGCTTCTGCTCAGCGTCCGCTGGACGAACTGGTTGAACAGGGTCTGTTCCGTCGTGACCTGTTCTTCCGTCTGAACGTTCTGACCCTGCAACTGCCGGCTCTGCGTAAACGTCGTGAACAGATCCTGCCGCTGTTCGACCAGTTCACCCAGGACGTTGCTGCTGAATCTGGTCGTTCTGTTCCGACCCTGGACAACCGTCGTGTTCAGATCCTGCTGTCTCACGACTGGCCGGGTAACGTTCGTGAACTGAAATCTGCTGCTAAACGTTTCGTTCTGGGTCTGCCGCTGCTGGGTGCTGAACCGGTTGAAGCTCGTGACCCGGTTACCGGTCTGCGTATGCAGATGCGTGTTATCGAAAAAATGCTGATCCAGGACGCTCTGAAACGTCACCGTCACAACTTCGACGCTGTTCTGGAAGAACTGGAACTGCCGCGTCGTACCCTGTACCACCGTATGAAAGAACTGGGTGTTGCTTCTCACATCGACCTGGTTGCTGAATCTTAATAA
+<p></p>TAATAA double stop codon added.
+<p></p>Codon optimization for E.coli was done using  [http://www.jcat.de/ JCat]  an online Codon Adaptation Tool which was published in NAR (Nucleic Acids Research).
+<p></p>Codon optimization results was present on  [http://2013.igem.org/Team:HIT-Harbin/Parts our wiki]
 ===Source===
-E.coli
+<p></p>[http://www.ncbi.nlm.nih.gov/nuccore/NC_007005.1?report=genbank&from=1351297&to=1352241 GeneBank NC_007005.1]
+<p></p>[https://parts.igem.org/wiki/index.php/Part:BBa_M39015 BBa_M39015]
 ===References===
+<p></p>[http://www.pnas.org/content/102/31/11064.short  Proc. Natl. Acad. Sci. U.S.A. 102 (31), 11064-11069 (2005)]
+<p></p>[http://nar.oxfordjournals.org/content/33/suppl_2/W526.short Andreas Grote et al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host]