Difference between revisions of "Part:BBa K1159003"

Latest revision as of 15:29, 12 October 2013

Engineered Fluorescein-Binding Anticalin FluA (triple mutant variant) in RFC[25]

Engineered version (triple mutant) of fluorescein-binding anticalin FluA with high binding affinity for fluorescein. This part is flanked by RFC[25] pre- and suffix for further protein fusions. This is an improved version of BBa_K157004.

The fluoresceine binding Anticalin FluA was in 2013 already present in the Parts Registry in RFC 25. As this BioBrick is from 2008 we used a higher engineered variant with three additional mutations which make it 75-times more affine to fluorescein (KD from 152 nM to 2 nM http://www.ncbi.nlm.nih.gov/pubmed/16307475 Vopel, Mühlbach and Skerra 2005). This seemed an imporatant point as this Anticalin was twice present in the finals of iGEM (Freiburg Team 2008 and 2009) and an improved variant may be helpful for further iGEM teams as well as for our purpose to use it as a binding protein on trangenic moss. The outdated FluA with a lower affinity was BBa_K157004.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 248
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Protein data table for BioBrick BBa_ automatically created by the BioBrick-AutoAnnotator version 1.0

Nucleotide sequence in RFC 25 N-Part using the stop codon in the suffix, so ACCGGT was added (in italics) to the 3' end: (underlined part encodes the protein)
AGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGACG ... AGGGTGAAGACCGGT
ORF from nucleotide position 53 to 106 (excluding stop-codon)

Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)

1	MVTLMGTNFLSVERVKTG*

Sequence features: (with their position in the amino acid sequence, see the list of supported features)

None of the supported features appeared in the sequence

Amino acid composition:

Ala (A)	0 (0.0%)
Arg (R)	1 (5.6%)
Asn (N)	1 (5.6%)
Asp (D)	0 (0.0%)

Cys (C)	0 (0.0%)
Gln (Q)	0 (0.0%)
Glu (E)	1 (5.6%)
Gly (G)	2 (11.1%)

His (H)	0 (0.0%)
Ile (I)	0 (0.0%)
Leu (L)	2 (11.1%)
Lys (K)	1 (5.6%)

Met (M)	2 (11.1%)
Phe (F)	1 (5.6%)
Pro (P)	0 (0.0%)
Ser (S)	1 (5.6%)

Thr (T)	3 (16.7%)
Trp (W)	0 (0.0%)
Tyr (Y)	0 (0.0%)
Val (V)	3 (16.7%)

Amino acid counting

	Total number:	18
	Positively charged (Arg+Lys):	2 (11.1%)
	Negatively charged (Asp+Glu):	1 (5.6%)
	Aromatic (Phe+His+Try+Tyr):	1 (5.6%)

Biochemical parameters

	Atomic composition:	C₈₆H₁₄₇N₂₃O₂₆S₂
	Molecular mass [Da]:	1983.4
	Theoretical pI:	8.50
	Extinction coefficient at 280 nm [M^-1 cm^-1]:	0 / 0 (all Cys red/ox)

Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges

Codon usage

	Organism:	E. coli	B. subtilis	S. cerevisiae	A. thaliana	P. patens	Mammals
	Codon quality (CAI):	good (0.74)	good (0.75)	good (0.61)	good (0.68)	excellent (0.83)	good (0.74)

Alignments (obtained from PredictProtein.org)
There were no alignments for this protein in the data base. The BLAST search was initialized and should be ready in a few hours.

Predictions (obtained from PredictProtein.org)

There were no predictions for this protein in the data base. The prediction was initialized and should be ready in a few hours.

The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For more information please see the documentation.
If you have any questions, comments or suggestions, please leave us a comment.

References

http://www.ncbi.nlm.nih.gov/pubmed/16307475 Vopel et al., 2005 Vopel, S., Mühlbach, H., Skerra, A.(2005) Rational engineering of a fluorescein-binding anticalin for improved ligand affinity. Biol Chem., 386(11):1097-104.

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1159003 short</partinfo>
-<html>
+Engineered version (triple mutant) of fluorescein-binding anticalin FluA with high binding affinity for fluorescein. This part is flanked by RFC[25] pre- and suffix for further protein fusions. This is an improved version of <partinfo>BBa_K157004</partinfo>.
-<table border="1" cellspacing="0" width="100%"><tr><td colspan="20"><strong>Automatically determined parameters using the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">BioBrick-AutoAnnotator</a></strong></td></tr><tr><td colspan="20"><strong>Nucleotide sequence:</strong> (underlined part of entry was translated, italic parts were added)<br>GTACACA<u>ATGCGTCGT...TTCGAAAAA</u>TAA</tr><tr><td colspan="20"><strong>Amino acid sequence:</strong><br><span style="font-family:'Courier New', Arial;">MRRSANYQPSIWDHDFLQSLNSNYTDEAYKRRAEELRGKVKIAIKDVIEPLDQLDLIDNLQRLGLAHRFETEIRNILNNIYNNNKDYNWRKENLYATSLE<br>FRLLRQHGYPVSQEVFNGFKDDQGGFICDDFKGILSLHEASYYSLEGESIMEEAWQFTSKHLKEVMISKNMEEDVFVAEQAKRALELPLHWKVPMLEARW<br>FIHIYERREDKNHLLLELAKMEFNTLQAIYQEELKEISGWWKDTGLGEKLSFARNRLVASFLWSMGIAFEPQFAYCRRVLTISIALITVIDDIYDVYGTL<br>DELEIFTDAVERWDINYALKHLPGYMKMCFLALYNFVNEFAYYVLKQQDFDLLLSIKNAWLGLIQAYLVEAKWYHSKYTPKLEEYLENGLVSITGPLIIT<br>ISYLSGTNPIIKKELEFLESNPDIVHWSSKIFRLQDDLGTSSDEIQRGDVPKSIQCYMHETGASEEVARQHIKDMMRQMWKKVNAYTADKDSPLTGTTTE<br>FLLNLVRMSHFMYLHGDGHGVQNQETIDVGFTLLFQPIPLEDKHMAFTASPGTKGTGAWSHPQFEK<br><br></span></tr><tr><td colspan="10">BioBrick: <partinfo>BBa_K801060</partinfo></td><td colspan="10">Used open reading frame from position 8 to 1705 (excluding stop-codon; if appropriate prefix/suffix were added).</td></tr><tr><td colspan="2">A (Ala)</td><td colspan="2">33 (5.83%)</td><td colspan="2">R (Arg)</td><td colspan="2">25 (4.42%)</td><td colspan="2">N (Asn)</td><td colspan="2">27 (4.77%)</td><td colspan="2">D (Asp)</td><td colspan="2">34 (6.01%)</td><td colspan="2">C (Cys)</td><td colspan="2">4 (0.71%)</td></tr><tr><td colspan="2">Q (Gln)</td><td colspan="2">24 (4.24%)</td><td colspan="2">E (Glu)</td><td colspan="2">48 (8.48%)</td><td colspan="2">G (Gly)</td><td colspan="2">29 (5.12%)</td><td colspan="2">H (His)</td><td colspan="2">18 (3.18%)</td><td colspan="2">I (Ile)</td><td colspan="2">39 (6.89%)</td></tr><tr><td colspan="2">L (Leu)</td><td colspan="2">64 (11.31%)</td><td colspan="2">K (Lys)</td><td colspan="2">36 (6.36%)</td><td colspan="2">M (Met)</td><td colspan="2">16 (2.83%)</td><td colspan="2">F (Phe)</td><td colspan="2">28 (4.95%)</td><td colspan="2">P (Pro)</td><td colspan="2">17 (3.00%)</td></tr><tr><td colspan="2">S (Ser)</td><td colspan="2">33 (5.83%)</td><td colspan="2">T (Thr)</td><td colspan="2">26 (4.59%)</td><td colspan="2">W (Trp)</td><td colspan="2">14 (2.47%)</td><td colspan="2">Y (Tyr)</td><td colspan="2">27 (4.77%)</td><td colspan="2">V (Val)</td><td colspan="2">24 (4.24%)</td></tr><tr><td colspan="2"><strong>Amino acid counting:</strong></td><td colspan="4">Total number of amino acids (aa):</td><td colspan="2">566</td><td colspan="4">Number of positively charged aa (Arg + Lys):</td><td colspan="2">61</td><td colspan="4">Number of negatively charged aa (Asp + Glu):</td><td colspan="2">82</td></tr><tr><td colspan="2"><strong>Biochemical parameters:</strong></td><td colspan="4">Molecular weight [Da]:</td><td colspan="2">66105.33</td><td colspan="4">Theoretical pI:</td><td colspan="2">5.38</td><td colspan="4">Extinction coefficient: [M^-1 cm^-1]</td><td colspan="2">117480 (all Cys as cystine), 117230 (no Cys as cystine)</td></tr><tr><td colspan="2"><strong>Estimated half-life:</strong></td><td colspan="4"><i>Mammals:</i></td><td colspan="2">30 hour</td><td colspan="4"><i>Yeast:</i></td><td colspan="2">>20 hour</td><td colspan="4"><i>E. coli</i>:</td><td colspan="2">>10 hour</td></tr><tr><td colspan="2"><strong>Codon usage:</strong> (CAI)</td><td colspan="4"><i>Mammals:</i></td><td colspan="2">0.68</td><td colspan="4"><i>Yeast:</i></td><td colspan="2">0.69</td><td colspan="4"><i>E. coli</i>:</td><td colspan="2">0.71</td></tr><tr><td colspan="3"><strong>RFC standard:</strong></td><td colspan="17">This is a RFC 10 BioBrick, nothing was added</td></tr><tr><td colspan="20"> The BioBrick-AutoAnnotator was created by <a href="http://2013.igem.org/Team:TU-Munich">TU-Munich 2013</a> iGEM team. For information please read the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">description</a>.</td></tr></table><br></html>
-<!-- Add more about the biology of this part here
+The fluoresceine binding Anticalin FluA was in 2013 already present in the Parts Registry in RFC 25. As this BioBrick is from 2008 we used a higher engineered variant with three additional mutations which make it 75-times more affine to fluorescein (KD from 152 nM to 2 nM [[http://www.ncbi.nlm.nih.gov/pubmed/16307475 Vopel, Mühlbach and Skerra 2005]]). This seemed an imporatant point as this Anticalin was twice present in the finals of iGEM (Freiburg Team 2008 and 2009) and an improved variant may be helpful for further iGEM teams as well as for our purpose to use it as a binding protein on trangenic moss. The outdated FluA with a lower affinity was BBa_K157004.
 ===Usage and Biology===
@@ Line 14: / Line 11: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1159003 SequenceAndFeatures</partinfo>
+<html><!--- Please copy this table containing parameters for BBa_ at the end of the parametrs section ahead of the references. ---><style type="text/css">table#AutoAnnotator {border:1px solid black; width:100%; border-collapse:collapse;} th#AutoAnnotatorHeader { border:1px solid black; width:100%; background-color: rgb(221, 221, 221);} td.AutoAnnotator1col { width:100%; border:1px solid black; } span.AutoAnnotatorSequence { font-family:'Courier New', Arial; } td.AutoAnnotatorSeqNum	{ text-align:right; width:2%; } td.AutoAnnotatorSeqSeq { width:98% } td.AutoAnnotatorSeqFeat1 { width:3% } td.AutoAnnotatorSeqFeat2a { width:27% } td.AutoAnnotatorSeqFeat2b { width:97% } td.AutoAnnotatorSeqFeat3 { width:70% } table.AutoAnnotatorNoBorder { border:0px; width:100%; border-collapse:collapse; } table.AutoAnnotatorWithBorder { border:1px solid black; width:100%; border-collapse:collapse; } td.AutoAnnotatorOuterAmino { border:0px solid black; width:20% } td.AutoAnnotatorInnerAmino { border:1px solid black; width:50% } td.AutoAnnotatorAminoCountingOuter { border:1px solid black; width:40%;  } td.AutoAnnotatorBiochemParOuter { border:1px solid black; width:60%; } td.AutoAnnotatorAminoCountingInner1 { width: 7.5% } td.AutoAnnotatorAminoCountingInner2 { width:62.5% } td.AutoAnnotatorAminoCountingInner3 { width:30% } td.AutoAnnotatorBiochemParInner1 { width: 5% } td.AutoAnnotatorBiochemParInner2 { width:55% } td.AutoAnnotatorBiochemParInner3 { width:40% } td.AutoAnnotatorCodonUsage1 { width: 3% } td.AutoAnnotatorCodonUsage2 { width:14.2% } td.AutoAnnotatorCodonUsage3 { width:13.8% } td.AutoAnnotatorAlignment1 { width: 3% } td.AutoAnnotatorAlignment2 { width: 10% } td.AutoAnnotatorAlignment3 { width: 87% } td.AutoAnnotatorLocalizationOuter {border:1px solid black; width:40%} td.AutoAnnotatorGOOuter {border:1px solid black; width:60%} td.AutoAnnotatorLocalization1 { width: 7.5% } td.AutoAnnotatorLocalization2 { width: 22.5% } td.AutoAnnotatorLocalization3 { width: 70% } td.AutoAnnotatorGO1 { width: 5% } td.AutoAnnotatorGO2 { width: 35% } td.AutoAnnotatorGO3 { width: 60% } td.AutoAnnotatorPredFeat1 { width:3% } td.AutoAnnotatorPredFeat2a { width:27% } td.AutoAnnotatorPredFeat3 { width:70% } div.AutoAnnotator_trans { position:absolute; background:rgb(11,140,143); background-color:rgba(11,140,143, 0.8); height:5px; top:100px; } div.AutoAnnotator_sec_helix { position:absolute; background:rgb(102,0,102); background-color:rgba(102,0,102, 0.8); height:5px; top:110px; } div.AutoAnnotator_sec_strand { position:absolute; background:rgb(245,170,26); background-color:rgba(245,170,26, 1); height:5px; top:110px; } div.AutoAnnotator_acc_buried { position:absolute; background:rgb(89,168,15); background-color:rgba(89,168,15, 0.8); height:5px; top:120px; } div.AutoAnnotator_acc_exposed { position:absolute; background:rgb(0, 0, 255); background-color:rgba(0, 0, 255, 0.8); height:5px; top:120px; } div.AutoAnnotator_dis { position:absolute; text-align:center; font-family:Arial,Helvetica,sans-serif; background:rgb(255, 200, 0); background-color:rgba(255, 200, 0, 1); height:16px; width:16px; top:80px; border-radius:50%; } </style><div id='AutoAnnotator_container_1381239135070'><table id="AutoAnnotator"><tr><!-- Time stamp in ms since 1/1/1970 1381239135070 --><th id="AutoAnnotatorHeader" colspan="2">Protein data table for BioBrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_<!------------------------Enter BioBrick number here------------------------>">BBa_<!------------------------Enter BioBrick number here------------------------></a> automatically created by the <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">BioBrick-AutoAnnotator</a> version 1.0</th></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Nucleotide sequence</strong> in <strong>RFC 25 N-Part</strong> using the stop codon in the suffix, so ACCGGT was added (in italics) to the 3' end: (underlined part encodes the protein)<br><span class="AutoAnnotatorSequence">&nbsp;AGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAG<u>ATGGTGACG&nbsp;...&nbsp;AGGGTGAAG<i>ACCGGT</i></u></span><br>&nbsp;<strong>ORF</strong> from nucleotide position 53 to 106 (excluding stop-codon)</td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Amino acid sequence:</strong> (RFC 25 scars in shown in bold, other sequence features underlined; both given below)<br><span class="AutoAnnotatorSequence"><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorSeqNum">1&nbsp;</td><td class="AutoAnnotatorSeqSeq">MVTLMGTNFLSVERVKTG*</td></tr></table></span></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Sequence features:</strong> (with their position in the amino acid sequence, see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software/FeatureList">list of supported features</a>)<table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorSeqFeat1"></td><td class="AutoAnnotatorSeqFeat2b">None of the supported features appeared in the sequence</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Amino acid composition:</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Ala (A)</td><td class="AutoAnnotatorInnerAmino">0 (0.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Arg (R)</td><td class="AutoAnnotatorInnerAmino">1 (5.6%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Asn (N)</td><td class="AutoAnnotatorInnerAmino">1 (5.6%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Asp (D)</td><td class="AutoAnnotatorInnerAmino">0 (0.0%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Cys (C)</td><td class="AutoAnnotatorInnerAmino">0 (0.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Gln (Q)</td><td class="AutoAnnotatorInnerAmino">0 (0.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Glu (E)</td><td class="AutoAnnotatorInnerAmino">1 (5.6%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Gly (G)</td><td class="AutoAnnotatorInnerAmino">2 (11.1%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">His (H)</td><td class="AutoAnnotatorInnerAmino">0 (0.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Ile (I)</td><td class="AutoAnnotatorInnerAmino">0 (0.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Leu (L)</td><td class="AutoAnnotatorInnerAmino">2 (11.1%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Lys (K)</td><td class="AutoAnnotatorInnerAmino">1 (5.6%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Met (M)</td><td class="AutoAnnotatorInnerAmino">2 (11.1%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Phe (F)</td><td class="AutoAnnotatorInnerAmino">1 (5.6%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Pro (P)</td><td class="AutoAnnotatorInnerAmino">0 (0.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Ser (S)</td><td class="AutoAnnotatorInnerAmino">1 (5.6%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Thr (T)</td><td class="AutoAnnotatorInnerAmino">3 (16.7%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Trp (W)</td><td class="AutoAnnotatorInnerAmino">0 (0.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Tyr (Y)</td><td class="AutoAnnotatorInnerAmino">0 (0.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Val (V)</td><td class="AutoAnnotatorInnerAmino">3 (16.7%)</td></tr></table></td></tr></table></td></tr><tr><td class="AutoAnnotatorAminoCountingOuter"><strong>Amino acid counting</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Total number:</td><td class="AutoAnnotatorAminoCountingInner3">18</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Positively charged (Arg+Lys):</td><td class="AutoAnnotatorAminoCountingInner3">2 (11.1%)</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Negatively charged (Asp+Glu):</td><td class="AutoAnnotatorAminoCountingInner3">1 (5.6%)</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Aromatic (Phe+His+Try+Tyr):</td><td class="AutoAnnotatorAminoCountingInner3">1 (5.6%)</td></tr></table></td><td class="AutoAnnotatorBiochemParOuter"><strong>Biochemical parameters</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Atomic composition:</td><td class="AutoAnnotatorBiochemParInner3">C<sub>86</sub>H<sub>147</sub>N<sub>23</sub>O<sub>26</sub>S<sub>2</sub></td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Molecular mass [Da]:</td><td class="AutoAnnotatorBiochemParInner3">1983.4</td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Theoretical pI:</td><td class="AutoAnnotatorBiochemParInner3">8.50</td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Extinction coefficient at 280 nm [M<sup>-1</sup> cm<sup>-1</sup>]:</td><td class="AutoAnnotatorBiochemParInner3">0 / 0 (all Cys red/ox)</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges</strong>&nbsp;<input type='button' id='hydrophobicity_charge_button' onclick='show_or_hide_plot_1381239135070()' value='Show'><span id="hydrophobicity_charge_explanation"></span><div id="hydrophobicity_charge_container" style='display:none'><div id="hydrophobicity_charge_placeholder0" style="width:100%;height:150px"></div></div></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Codon usage</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorCodonUsage1"></td><td class="AutoAnnotatorCodonUsage2">Organism:</td><td class="AutoAnnotatorCodonUsage3"><i>E. coli</i></td><td class="AutoAnnotatorCodonUsage3"><i>B. subtilis</i></td><td class="AutoAnnotatorCodonUsage3"><i>S. cerevisiae</i></td><td class="AutoAnnotatorCodonUsage3"><i>A. thaliana</i></td><td class="AutoAnnotatorCodonUsage3"><i>P. patens</i></td><td class="AutoAnnotatorCodonUsage3">Mammals</td></tr><tr><td class="AutoAnnotatorCodonUsage1"></td><td class="AutoAnnotatorCodonUsage2">Codon quality (<a href="http://en.wikipedia.org/wiki/Codon_Adaptation_Index">CAI</a>):</td><td class="AutoAnnotatorCodonUsage3">good (0.74)</td><td class="AutoAnnotatorCodonUsage3">good (0.75)</td><td class="AutoAnnotatorCodonUsage3">good (0.61)</td><td class="AutoAnnotatorCodonUsage3">good (0.68)</td><td class="AutoAnnotatorCodonUsage3">excellent (0.83)</td><td class="AutoAnnotatorCodonUsage3">good (0.74)</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Alignments</strong> (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)<br>&nbsp;&nbsp;&nbsp;There were no alignments for this protein in the data base. The BLAST search was initialized and should be ready in a few hours.</td></tr><tr><th id='AutoAnnotatorHeader' colspan="2"><strong>Predictions</strong> (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)</th></tr><tr><td class="AutoAnnotator1col" colspan="2">&nbsp;&nbsp;&nbsp;There were no predictions for this protein in the data base. The prediction was initialized and should be ready in a few hours.</td><tr><td class="AutoAnnotator1col" colspan="2"> The BioBrick-AutoAnnotator was created by <a href="http://2013.igem.org/Team:TU-Munich">TU-Munich 2013</a> iGEM team. For more information please see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">documentation</a>.<br>If you have any questions, comments or suggestions, please leave us a <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">comment</a>.</td></tr></table></div><br><!-- IMPORTANT: DON'T REMOVE THIS LINE, OTHERWISE NOT SUPPORTED FOR IE BEFORE 9 --><!--[if lte IE 8]><script language="javascript" type="text/javascript" src="excanvas.min.js"></script><![endif]--><script type='text/javascript' src='http://code.jquery.com/jquery-1.10.0.min.js'></script><script type='text/javascript' src='http://2013.igem.org/Team:TU-Munich/Flot.js?action=raw&ctype=text/js'></script><script>function show_or_hide_plot_1381239135070(){hydrophobicity_datapoints = [[2.5,2.22],[3.5,1.76],[4.5,0.78],[5.5,0.22],[6.5,0.02],[7.5,0.40],[8.5,0.32],[9.5,1.30],[10.5,1.30],[11.5,-0.16],[12.5,-0.08],[13.5,-0.70],[14.5,-1.68],[15.5,-1.06]];charge_datapoints = 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 <!-- Uncomment this to enable Functional Parameter display
@@ Line 20: / Line 17: @@
 <partinfo>BBa_K1159003 parameters</partinfo>
 <!-- -->
+==References==
+----
+*[[http://www.ncbi.nlm.nih.gov/pubmed/16307475 Vopel et al., 2005]] Vopel, S., Mühlbach, H., Skerra, A.(2005) Rational engineering of a fluorescein-binding anticalin for improved ligand affinity. ''Biol Chem.'', 386(11):1097-104.