Difference between revisions of "Part:BBa K1051300"

 
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1051300 short</partinfo>
 
<partinfo>BBa_K1051300 short</partinfo>
−
 
+
<h3>Purpose</h3>
−
It is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the cln2 sequence before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the  metabolic product of the Bacterial you use for transformation .
+
Works for the cell synchronization device, activates Cdc28p kinase to promote the G1 to S phase transition
 +
<h3>Principle</h3>
 +
"CLn2" is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the cln2 sequence before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the  metabolic product of the Bacterial you use for transformation .<br />
 +
Experimental results show that it activates Cdc28p kinase to promote the G1 to S phase transition .
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 17: Line 19:
 
<partinfo>BBa_K1051300 parameters</partinfo>
 
<partinfo>BBa_K1051300 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
<br />
 +
<!-- Add more about the biology of this part her
 +
===Usage and Biology===

Latest revision as of 16:26, 27 September 2013

cln2 promoter (G1 phase to S phase)

Purpose

Works for the cell synchronization device, activates Cdc28p kinase to promote the G1 to S phase transition

Principle

"CLn2" is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the cln2 sequence before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation .
Experimental results show that it activates Cdc28p kinase to promote the G1 to S phase transition .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 511