Difference between revisions of "Part:BBa J100111:Design"

 
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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This construct was built using iPCR primers to amplify the promoter/RBS/eCDM8 construct (J100101) on pSB1A8 and add to BsaI sites to the end of the amplified sequence. This amplification removed the stuffer and the XbaI site from pSB1A8. PCR primers were used to amplify the promoter/riboswitch/tetA construct (J119140) and to add BsaI sites to the end of this sequence. GGA was then used to ligate these two amplified parts together.  
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This construct was built using iPCR primers to amplify the promoter/RBS/eCDM8 construct (J100101) on pSB1A8 and add to BsaI sites to the end of the amplified sequence. This amplification removed the stuffer and the SpeI site from pSB1A8. PCR primers were used to amplify the promoter/riboswitch/tetA construct (J119140) and to add BsaI sites to the end of this sequence. GGA was then used to ligate these two amplified parts together.
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===Source===
 
===Source===

Latest revision as of 13:53, 28 June 2013


eCDM8 and Tetracycline Resistance Riboswitch into pSB1A8


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3578
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 237
    Illegal BamHI site found at 3724
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3750
    Illegal NgoMIV site found at 4118
    Illegal NgoMIV site found at 4278
    Illegal AgeI site found at 196
    Illegal AgeI site found at 857
    Illegal AgeI site found at 3372
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This construct was built using iPCR primers to amplify the promoter/RBS/eCDM8 construct (J100101) on pSB1A8 and add to BsaI sites to the end of the amplified sequence. This amplification removed the stuffer and the SpeI site from pSB1A8. PCR primers were used to amplify the promoter/riboswitch/tetA construct (J119140) and to add BsaI sites to the end of this sequence. GGA was then used to ligate these two amplified parts together.

Source

All parts taken from the Registry.

References