Difference between revisions of "Part:BBa K511801"
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__NOTOC__
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<partinfo>BBa_K511801 short</partinfo>
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This MammoBlock composite device produces the monomeric red fluorescent protein mKate at a low, constitutive level driven by the Hef1a promoter.
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<html><img src='https://static.igem.org/mediawiki/2011/0/0d/Specimen_001_Charles_2.fcs_scatter.jpg
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' style="width:60%"><br></html>
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<html><img src='https://static.igem.org/mediawiki/2011/1/12/Specimen_001_Charles_1.fcs_scatter.jpg
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' style="width:60%"><br></html>
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(1A) This scatter plot shows the distribution of the Hek293 population after it was transfected with the following DNA parts: Hef1a-LacO:eYFP and Hef1a:mKate, both of which were constitutively active, expressing yellow and red fluorescent proteins respectively. We observe a distinct shift of approximately 83% of the population in their eYFP fluorescence (FITC channel), while we observe a 69% shift in mKate fluorescence (PE-TexasRed channel). (1C) The negative control sample was transfected with plasmids containing no functional promotor-gene pairs.  
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K511801 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K511801 parameters</partinfo>
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<!-- -->

Latest revision as of 16:18, 10 May 2013

Red Fluorescent Protein Generator (Hef1a-mKate) MammoBlock Device

This MammoBlock composite device produces the monomeric red fluorescent protein mKate at a low, constitutive level driven by the Hef1a promoter.



(1A) This scatter plot shows the distribution of the Hek293 population after it was transfected with the following DNA parts: Hef1a-LacO:eYFP and Hef1a:mKate, both of which were constitutively active, expressing yellow and red fluorescent proteins respectively. We observe a distinct shift of approximately 83% of the population in their eYFP fluorescence (FITC channel), while we observe a 69% shift in mKate fluorescence (PE-TexasRed channel). (1C) The negative control sample was transfected with plasmids containing no functional promotor-gene pairs.

Sequence and Features


Assembly Compatibility:
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    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
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    INCOMPATIBLE WITH RFC[21]
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    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
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    Illegal PstI site found at 866
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    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1235
    Illegal PstI site found at 361
    Illegal PstI site found at 866
    Illegal NgoMIV site found at 749
    Illegal AgeI site found at 127
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1899
    Illegal SapI.rc site found at 1281