Difference between revisions of "Part:BBa M36098:Design"
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===References=== | ===References=== | ||
+ | US Patent Number: US008138324 | ||
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+ | Biotechniques. 2006 Mar;40(3):355-64. | ||
+ | Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system. | ||
+ | Giacalone MJ, Gentile AM, Lovitt BT, Berkley NL, Gunderson CW, Surber MW. |
Latest revision as of 20:03, 25 April 2013
E. coli actuator test rig
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1995
Illegal EcoRI site found at 2098
Illegal XbaI site found at 2047 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1995
Illegal EcoRI site found at 2098
Illegal NheI site found at 286 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1995
Illegal EcoRI site found at 2098
Illegal BglII site found at 1315
Illegal BamHI site found at 2053 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1995
Illegal EcoRI site found at 2098
Illegal XbaI site found at 2047 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1995
Illegal EcoRI site found at 2098
Illegal XbaI site found at 2047
Illegal AgeI site found at 1583 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI site found at 2330
Design Notes
DNA2.0 licenses this plasmid from Xbrane and charges for its use.
Source
Xbrane is has a patent on this plasmid, Patent Number: US008138324.
References
US Patent Number: US008138324
Biotechniques. 2006 Mar;40(3):355-64. Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system. Giacalone MJ, Gentile AM, Lovitt BT, Berkley NL, Gunderson CW, Surber MW.