Difference between revisions of "Part:BBa M36099:Design"
(3 intermediate revisions by the same user not shown) | |||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_M36099 short</partinfo> | <partinfo>BBa_M36099 short</partinfo> | ||
Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
− | + | Cloning Cassettes 1 and 2 used for inserting parts into an empty vector. Parts in between the cloning cassettes were designed by BIOE44. The parts including and outside of the cloning cassettes were provided by DNA2.0. | |
− | + | ||
+ | rpo_bla and rrnB1T1T2 transcription terminators serve as insulators. | ||
===Source=== | ===Source=== | ||
− | + | Comet GFP from DNA2.0 The rest of the vector is sourced from DNA2.0. | |
===References=== | ===References=== |
Latest revision as of 21:39, 14 January 2015
E. coli sensor test rig
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 2342
Illegal XbaI site found at 3188 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 2342
Illegal XbaI site found at 3188 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 2342
Illegal XbaI site found at 3188
Illegal AgeI site found at 2605 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Design Notes
Cloning Cassettes 1 and 2 used for inserting parts into an empty vector. Parts in between the cloning cassettes were designed by BIOE44. The parts including and outside of the cloning cassettes were provided by DNA2.0.
rpo_bla and rrnB1T1T2 transcription terminators serve as insulators.
Source
Comet GFP from DNA2.0 The rest of the vector is sourced from DNA2.0.