Difference between revisions of "Part:BBa J100109"

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__NOTOC__
 
__NOTOC__
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This CDS codes for the adhE gene from E. coli and can be used in conjunction with an adhE- strain for complementation. This has been designed to go downstream of a C-Dog RBS using golden gate assembly (GGA) with BsaI as the restriction enzyme.  
 
This CDS codes for the adhE gene from E. coli and can be used in conjunction with an adhE- strain for complementation. This has been designed to go downstream of a C-Dog RBS using golden gate assembly (GGA) with BsaI as the restriction enzyme.  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_H100109 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_H100109 parameters</partinfo>
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<partinfo>BBa_J100109 parameters</partinfo>
 
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Latest revision as of 14:43, 18 April 2013

adhE optimized for E. coli, PstI removed

This CDS codes for the adhE gene from E. coli and can be used in conjunction with an adhE- strain for complementation. This has been designed to go downstream of a C-Dog RBS using golden gate assembly (GGA) with BsaI as the restriction enzyme.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2691
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 2692
    Illegal PstI site found at 2706
    Illegal NotI site found at 2699
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 506
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2692
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 2692
    Illegal PstI site found at 2706
    Illegal AgeI site found at 591
    Illegal AgeI site found at 1815
    Illegal AgeI site found at 1860
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7