Difference between revisions of "Light"
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=Fluorescent Proteins= | =Fluorescent Proteins= | ||
[https://parts.igem.org/Featured_Parts:Fluorescent_proteins Featured Parts], | [https://parts.igem.org/Featured_Parts:Fluorescent_proteins Featured Parts], | ||
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=Photoproteins= | =Photoproteins= | ||
Aequorin, Obelin | Aequorin, Obelin | ||
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| + | =Split-BioBricks for protein interactions= | ||
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| + | =Degradation Tags for kinetic measurements= | ||
Latest revision as of 12:22, 7 March 2013
Contents
Fluorescent Proteins
Featured Parts, [http://www.microscopyu.com/articles/livecellimaging/fpintro.html Nikkon],
| Name | Exitation [nm] | Emmission [nm] | Quant. yield | Molar extinct. | BioBrick RFC10 | BioBrick RFC25 | Reference |
| Cerulean | 433 | 475 | 0.62 | 43000 | BBa_E2020 | ||
| GFPmut3b | 501 | 511 | BBa_E0040 | ||||
| amilGFP | 503 | 512 | 0.67 | 75200 | BBa_K592010 | [http://www.ncbi.nlm.nih.gov/nuccore/ay646067 GeneBank] | |
| mVenus | 515 | 528 | 0.57 | 92200 | BBa_I757008 | ||
| mOrange | 548 | 562 | 0.69 | 71000 | BBa_E2050 | ||
| mStrawbery | 574 | 596 | 0.29 | 90000 | BBa_K399001 | ||
| mRFP1 | 584 | 607 | 0.25 | 50000 | BBa_E1010 | ||
| mCherry | 587 | 610 | 0.22 | 72000 | BBa_E2060 | ||
| eforRed | 589 | 609 | 0.16 | 111300 | BBa_K592012 | [http://www.ncbi.nlm.nih.gov/nuccore/EU498726 GeneBank] | |
| cjBlue | 610 | BBa_K592011 | [http://www.ncbi.nlm.nih.gov/pubmed/17028187 PubMed] | ||||
| aeBlue | BBa_K864401 | [http://www.ncbi.nlm.nih.gov/pubmed/16164420 PubMed] | |||||
| amilCP | 588 | NA | NA | 8760 | BBa_K592009 | [http://www.ncbi.nlm.nih.gov/nuccore/AY646075 GeneBank] |
Luciferases
| Name | Organism | Substrate | pH optimum | Amino acids | Mol. mass [kDa] | Emmission [nm] | BioBrick RFC10 | BioBrick RFC25 | Reference |
| Photinus/Firefly luciferase | D-luciferin + ATP | 550 | 60.6 | 578 | BBa_I712019 | ||||
| Renilla luciferase | coelenterazine + O2 | 311 | 36.0 | 480 | BBa_J52008 | ||||
| Gaussia luciferase | coelenterazine + O2 | 189 | 20.3 | 480 | BBa_K509005 | ||||
| Nano luciferase | furimazine | 171 | 19.1 | 460 | [http://www.reportergene.com/2013/01/the-nanoluc-luciferase-reporter-dossier.html 1] |
Photoproteins
Aequorin, Obelin