Difference between revisions of "Test2"

(Fluorescent Proteins)
 
(37 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[image:Icon_Sensors.png]]
+
[[image:Icon_RNA.png ]]
 +
 
 +
 
 +
*Proteinsynthesis
 +
**Polyadenylation
 +
**Terminators
 +
 
 +
*Tags
 +
**His-Tag
 +
**Strep-tagII
 +
**HA-tag
 +
**GST-Tag
 +
 
 +
*Secretionsignals
 +
 
 +
 
  
  
Line 45: Line 60:
 
|align='center' width=80px |{{Click || image=Icon_Odor.png | link=Odor |width=50px | height=50px }}
 
|align='center' width=80px |{{Click || image=Icon_Odor.png | link=Odor |width=50px | height=50px }}
 
|'''[[Odor|Odor production and sensing]]''': Parts that produce or sense odorants aer listet here.
 
|'''[[Odor|Odor production and sensing]]''': Parts that produce or sense odorants aer listet here.
 +
|-
 +
|align='center' width=80px |{{Click || image=Icon_RNA.png | link=RNA |width=50px | height=50px }}
 +
|'''[[RNA]]''': Parts used as biosensors are listed here.
 
|-
 
|-
 
|align='center' width=80px |{{Click || image=Icon_Sensors.png | link=Sensors |width=50px | height=50px }}
 
|align='center' width=80px |{{Click || image=Icon_Sensors.png | link=Sensors |width=50px | height=50px }}
Line 57: Line 75:
  
 
<br><br><br><br><br><br><br><br><br>
 
<br><br><br><br><br><br><br><br><br>
 
=Fluorescent Proteins=
 
[https://parts.igem.org/Featured_Parts:Fluorescent_proteins Featured Parts],
 
[http://www.microscopyu.com/articles/livecellimaging/fpintro.html Nikkon],
 
[http://2012.igem.org/Team:Uppsala_University/Chromoproteins Chromoproteins Uppsala]
 
https://parts.igem.org/Part:BBa_K180009 mBanana
 
 
{|cellspacing="0" border="1"
 
|Name
 
|Exitation [nm]
 
|Emmission [nm]
 
|Quant. yield
 
|Molar extinct.
 
|BioBrick RFC10
 
|BioBrick RFC25
 
|Reference
 
|-
 
|style="background:#FFFF00" |Cerulean
 
|433
 
|475
 
|0.62
 
|43000
 
|<partinfo>BBa_E2020</partinfo>
 
|
 
|
 
|-
 
|style="background:#FFFF00" |GFPmut3b
 
|501
 
|511
 
|
 
|
 
|<partinfo>BBa_E0040</partinfo>
 
|
 
|
 
|-
 
|style="background:#FFFF00" |amilGFP
 
|503
 
|512
 
|0.67
 
|75200
 
|<partinfo>BBa_K592010</partinfo>
 
|
 
|[http://www.ncbi.nlm.nih.gov/nuccore/ay646067 GeneBank]
 
|-
 
|style="background:#FFFF00" |mVenus
 
|515
 
|528
 
|0.57
 
|92200
 
|
 
|<Partinfo>BBa_I757008</partinfo>
 
|
 
|-
 
|style="background:#FFFF00" |mOrange
 
|548
 
|562
 
|0.69
 
|71000
 
|<partinfo>BBa_E2050</partinfo>
 
|
 
|
 
|-
 
|style="background:#FFFF00" |mStrawbery
 
|574
 
|596
 
|0.29
 
|90000
 
|<partinfo>BBa_K399001</partinfo>
 
|
 
|
 
|-
 
|style="background:#8B0000" |mRFP1
 
|584
 
|607
 
|0.25
 
|50000
 
|<partinfo>BBa_E1010</partinfo>
 
|
 
|
 
|-
 
|style="background:#FFFF00" |mCherry
 
|587
 
|610
 
|0.22
 
|72000
 
|<partinfo>BBa_E2060</partinfo>
 
|
 
|
 
|-
 
|style="background:#FF34B3" |eforRed
 
|589
 
|609
 
|0.16
 
|111300
 
|<partinfo>BBa_K592012</partinfo>
 
|
 
|[http://www.ncbi.nlm.nih.gov/nuccore/EU498726 GeneBank]
 
|-
 
|style="background:#66CDAA" |cjBlue
 
|610
 
|
 
|
 
|
 
|<partinfo>BBa_K592011</partinfo>
 
|
 
|[http://www.ncbi.nlm.nih.gov/pubmed/17028187 PubMed]
 
|-
 
|style="background:#473C8B" |aeBlue
 
|
 
|
 
|
 
|
 
|<partinfo>BBa_K864401</partinfo>
 
|
 
|[http://www.ncbi.nlm.nih.gov/pubmed/16164420 PubMed]
 
|-
 
|style="background:#FFFF00" |amilCP
 
|588
 
|NA
 
|NA
 
|8760
 
|<partinfo>BBa_K592009</partinfo>
 
|
 
|[http://www.ncbi.nlm.nih.gov/nuccore/AY646075 GeneBank]
 
|}
 
<br>
 
 
=Fluciferases=
 
{|cellspacing="0" border="1"
 
|Name
 
|Photinus luciferase
 
|Renilla luciferase
 
|Gaussia luciferase
 
|Nano Luciferase
 
|-
 
|Reference
 
|
 
|
 
|
 
|[http://www.reportergene.com/2013/01/the-nanoluc-luciferase-reporter-dossier.html]
 
|-
 
|Organism
 
|
 
|
 
|
 
|
 
|-
 
|Substrate
 
|
 
|
 
|
 
|
 
|-
 
|pH optimum
 
|
 
|
 
|
 
|
 
|-
 
|Substrate
 
|
 
|
 
|
 
|
 
|-
 
|Amino acids
 
|
 
|
 
|
 
|
 
|-
 
|Molecular mass
 
|
 
|
 
|
 
|
 
|-
 
|BioBricks RFC 10
 
|
 
|
 
|
 
|
 
|-
 
|BioBricks RFC 25
 
|<partinfo>BBa_I712019</partinfo>
 
|<partinfo>BBa_J52008</partinfo>
 
|<partinfo>BBa_K509005</partinfo>
 
|
 
|}
 
 
=Photoproteins= (Aequorin, Obelin
 

Latest revision as of 14:54, 16 March 2013

Icon RNA.png


  • Proteinsynthesis
    • Polyadenylation
    • Terminators
  • Tags
    • His-Tag
    • Strep-tagII
    • HA-tag
    • GST-Tag
  • Secretionsignals



Biosafety: Parts improving biological safety, containment.
Biosynthesis: Parts involved in the production of chemicals and metabolites are listed here.
Biodegradation: Parts involved in the degradation of chemicals are listed here.
Cell-cell signaling and quorum sensing: Parts involved in intercellular signaling and quorum sensing between bacteria.
Cell death: Parts involved in killing cells.
Coliroid: Parts involved in taking a bacterial photograph.
Conjugation: Parts involved in DNA conjugation between bacteria.
DNA binding: Parts involved in xxx.
DNA recombination: Parts involved in DNA recombination are listet here.
Ions: Parts involved in sensing, transport and binding of different ions are listet in here.
Ligand Binding: Parts involved in binding chemical structures are listet here.
Light production and sensing: Parts involved in generation and sensing of light are listet here.
Motility and chemotaxis: Parts involved in motility or chemotaxis of cells are listet here.
Odor production and sensing: Parts that produce or sense odorants aer listet here.
RNA: Parts used as biosensors are listed here.
Sensors: Parts used as biosensors are listed here.
Viral vectors: Parts involved in the production and modification of Viral vectors are listet here..