Difference between revisions of "Part:BBa M36071:Design"

(→‎Design Notes)
 
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===References===
 
===References===
 +
 +
Durfee, Tim, et al. "The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse." Journal of bacteriology 190.7 (2008): 2597-2606.
 +
 +
Rui B, Shen T, Zhou H, Liu J, Chen J, et al. A systematic investigation of Escherichia coli central carbon
 +
metabolism in response to superoxide stress. BMC Syst Biol. 2010;4:122.
 +
 +
Keele Jr, Bernard B., J. M. McCord, and I. Fridovich. "Superoxide dismutase from Escherichia coli BA new
 +
manganese-containing enzyme." Journal of Biological Chemistry 245.22 (1970): 6176-6181.

Latest revision as of 11:35, 7 December 2012

K12 e.coli native Manganese Superoxide Dismutase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 346
    Illegal PstI site found at 283
    Illegal PstI site found at 593
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 346
    Illegal PstI site found at 283
    Illegal PstI site found at 593
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 346
    Illegal XhoI site found at 43
    Illegal XhoI site found at 127
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 346
    Illegal PstI site found at 283
    Illegal PstI site found at 593
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 346
    Illegal PstI site found at 283
    Illegal PstI site found at 593
    Illegal NgoMIV site found at 542
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This DNA sequence is converted from the amino acid sequence of Mn-SOD protein using the online converter from In-Silico. We optimized our sequence in DNA 2.0's Gene Designer by increasing GC content and minimizing repeats. We accomplished this by altering basepairs without altering amino acids.

Source

This sequence is derived from the amino acid sequence of Mn-SOD protein found from Durfee et. al at the University of Wisconsin which also provided the entire genomic sequence of the E. coli (strain: k-12, substrain: DH10B) genome.

References

Durfee, Tim, et al. "The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse." Journal of bacteriology 190.7 (2008): 2597-2606.

Rui B, Shen T, Zhou H, Liu J, Chen J, et al. A systematic investigation of Escherichia coli central carbon metabolism in response to superoxide stress. BMC Syst Biol. 2010;4:122.

Keele Jr, Bernard B., J. M. McCord, and I. Fridovich. "Superoxide dismutase from Escherichia coli BA new manganese-containing enzyme." Journal of Biological Chemistry 245.22 (1970): 6176-6181.