Difference between revisions of "Part:BBa K902041:Experience"
 
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<html><i>amdA</i> is an Amidase gene responsible for the removal of primary amides from a variety of compounds.  We validated the ability of this gene to remove the primary amide group from benzamide.  This was evaluated through GC/MS analysis and detailed on our <a href="http://2012.igem.org/Team:Calgary/Notebook/Protocols/AmidaseAssay">protocol page</a>.  This is detailed in the figures below:
 
<html><i>amdA</i> is an Amidase gene responsible for the removal of primary amides from a variety of compounds.  We validated the ability of this gene to remove the primary amide group from benzamide.  This was evaluated through GC/MS analysis and detailed on our <a href="http://2012.igem.org/Team:Calgary/Notebook/Protocols/AmidaseAssay">protocol page</a>.  This is detailed in the figures below:
  
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</html>[[Image:Calgary AmdA Chromatograph.png|thumb|450px|left|Figure 1: GC chromatograph demonstrating the degradation of benzamide by <i>AmdA</i> compared to an <i>E. coli</i> control.  Additionally, a smaller peak with an earlier retention time is produced solely in the sample run.]]<html>
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</html>[[Image:Calgary AmdA Chromatograph.png|thumb|400px|center|Figure 1: GC chromatograph demonstrating the degradation of benzamide by <i>AmdA</i> compared to an <i>E. coli</i> control.  Additionally, a smaller peak with an earlier retention time is produced solely in the sample run.]]<html>
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</html>[[Image:Calgary AmdA MSData.png|thumb|450px|right|Figure 2: Mass Spectra for the peak at a retention time of 10.5 minutes from Figure 1 representing the substrate benzamide.  Additionally, the peak at retention time of 9 minutes represents benzoic acid based on its mass suggesting that their is a small accumulation of product.]]
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</html>[[Image:Calgary AmdA MSData.png|thumb|400px|center|Figure 2: Mass Spectra for the peak at a retention time of 10.5 minutes from Figure 1 representing the substrate benzamide.  Additionally, the peak at retention time of 9 minutes represents benzoic acid based on its mass suggesting that their is a small accumulation of product.]]
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===User Reviews===
 
===User Reviews===

Latest revision as of 20:06, 26 October 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K902041

amdA is an Amidase gene responsible for the removal of primary amides from a variety of compounds. We validated the ability of this gene to remove the primary amide group from benzamide. This was evaluated through GC/MS analysis and detailed on our protocol page. This is detailed in the figures below:
Figure 1: GC chromatograph demonstrating the degradation of benzamide by AmdA compared to an E. coli control. Additionally, a smaller peak with an earlier retention time is produced solely in the sample run.
Figure 2: Mass Spectra for the peak at a retention time of 10.5 minutes from Figure 1 representing the substrate benzamide. Additionally, the peak at retention time of 9 minutes represents benzoic acid based on its mass suggesting that their is a small accumulation of product.




User Reviews

UNIQd0b090422f8ee319-partinfo-00000002-QINU UNIQd0b090422f8ee319-partinfo-00000003-QINU