Difference between revisions of "Part:BBa K902081"
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<partinfo>BBa_K902081 short</partinfo> | <partinfo>BBa_K902081 short</partinfo> | ||
− | + | This part produces HpaC and KatG-LAA under control of an inducible lacI promoter. | |
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+ | KatG is a catalase, and serves to detoxify hydrogen peroxide. HpaC is an oxidoreductase capable of recycling FMN to FMNH<sub>2</sub> while converting NADH to NAD. This part is used in conjuctions with the 4S desulfurization pathway to replace DszD. Doing so improves the rate of desulfurization 7-10x.<br> [[Image:File-Ucalgary team sulfur 4s enzyme pathway diagram.png|centre]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 23:49, 26 October 2012
4S Desulfurization Pathway Optimization Circuit (PlacI/hpaC/RBS/katGLAA)
This part produces HpaC and KatG-LAA under control of an inducible lacI promoter.
KatG is a catalase, and serves to detoxify hydrogen peroxide. HpaC is an oxidoreductase capable of recycling FMN to FMNH2 while converting NADH to NAD. This part is used in conjuctions with the 4S desulfurization pathway to replace DszD. Doing so improves the rate of desulfurization 7-10x.Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 993
Illegal BglII site found at 1928
Illegal BglII site found at 2055
Illegal BamHI site found at 1845 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 509