Difference between revisions of "Part:BBa K902080:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K902080 short</partinfo>
 
<partinfo>BBa_K902080 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
mutation
+
DszB has had a point mutation introduced with site-directed mutagenesis to increase activity (Y63F)- see the part page for DszB for more info
 +
 
 +
<i>dszB</i> and <i>dszC</i> were made biobrick compatible through site directed mutagenesis to remove illegal cut sites- see the part pages for more info
  
  
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===Source===
 
===Source===
  
−
Rhodococcus
+
Assembled from biobrick parts using basic biobrick assembly method.
  
 
===References===
 
===References===
 +
Oshiro T, Ohkita R, Takikawa T, Manabe M, Lee WC, Tanokura M, Izumi Y. Improvement of 2'-hydroxybiphenyl-2-sulfinate desulfinase, an enzyme involved in the dibenzothiophene desulfurization pathway, from <i>Rhodococcus erythropolis</i> KA2-5-1 by site-directed mutagenesis. Biosci Biotechnol Biochem. 2007 Nov.; 71(11):2815-21

Latest revision as of 03:57, 27 October 2012

PlacI/dszB/B0034/dszC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1428
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 652
    Illegal BamHI site found at 2232
    Illegal XhoI site found at 644
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 359
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2382


Design Notes

DszB has had a point mutation introduced with site-directed mutagenesis to increase activity (Y63F)- see the part page for DszB for more info

dszB and dszC were made biobrick compatible through site directed mutagenesis to remove illegal cut sites- see the part pages for more info


Source

Assembled from biobrick parts using basic biobrick assembly method.

References

Oshiro T, Ohkita R, Takikawa T, Manabe M, Lee WC, Tanokura M, Izumi Y. Improvement of 2'-hydroxybiphenyl-2-sulfinate desulfinase, an enzyme involved in the dibenzothiophene desulfurization pathway, from Rhodococcus erythropolis KA2-5-1 by site-directed mutagenesis. Biosci Biotechnol Biochem. 2007 Nov.; 71(11):2815-21