Difference between revisions of "Part:BBa K801074:Design"
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<!--*used_abbreviation_1 = full_name_of_used_abbreviations_1--> | <!--*used_abbreviation_1 = full_name_of_used_abbreviations_1--> | ||
<!--*used_abbreviation_2 = full_name_of_used_abbreviations_2--> | <!--*used_abbreviation_2 = full_name_of_used_abbreviations_2--> | ||
| + | * CaMXMT1 = coffea arabica 7-methylxanthine N-methyltransferase 1 | ||
===Design Notes=== | ===Design Notes=== | ||
'''Related BioBrick:''' | '''Related BioBrick:''' | ||
| − | + | [https://parts.igem.org/Part:BBa_K801074 BBa_K801071] | |
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] --> | <!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] --> | ||
<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] | <!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] | ||
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'''Source:'''<br> | '''Source:'''<br> | ||
| + | *Preexisting BioBrick BBa_K801071 | ||
<!--*Commercial system: plasmid name, system name, company name--> | <!--*Commercial system: plasmid name, system name, company name--> | ||
<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?--> | <!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?--> | ||
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'''Organism:'''<br> | '''Organism:'''<br> | ||
| + | * Genesequence derived from ''Coffea arabica'' | ||
| + | * codonoptimized for ''Saccharomyces cerevisiae'' | ||
| + | * designed for ''Saccharomyces cerevisiae'' | ||
<!--*Genesequence derived from ''?organism_name?''--> | <!--*Genesequence derived from ''?organism_name?''--> | ||
<!--*Codonoptimized for ''?organism_name?''--> | <!--*Codonoptimized for ''?organism_name?''--> | ||
<!--*Designed for the following Chassis: ''?organism-name?''--> | <!--*Designed for the following Chassis: ''?organism-name?''--> | ||
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===References=== | ===References=== | ||
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'''Literature references:'''<br> | '''Literature references:'''<br> | ||
| + | *[http://www.ncbi.nlm.nih.gov/pubmed/12746542 '''Pubmed:''' Uefuji H., Ogita S., Yamaguchi Y., Koizumi N. and Sano H., 2003: Molecular cloning and functional characterization of three distinct n-methyltransferases involved in the caffeine biosynthetic pathway in coffee plants.] | ||
| + | *[http://www.ncbi.nlm.nih.gov/pubmed/16247553 '''Pubmed:''' Uefuji H., Ogita S., Yamaguchi Y., Koizumi N. and Sano H., 2005: Caffeine production in tobacco plants by simultaneous expression of three coffee N-methyltrasferases and its potential as a pest repellant.] | ||
| + | *[http://www.ncbi.nlm.nih.gov/pubmed/16925551 '''Pubmed:''' Kuranda K, Leberre V, Sokol S, Palamarczyk G, François J., 2006: Investigating the caffeine effects in the yeast Saccharomyces cerevisiae brings new insights into the connection between TOR, PKC and Ras/cAMP signalling pathways.] | ||
<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]--> | <!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]--> | ||
'''Database references:'''<br> | '''Database references:'''<br> | ||
| + | * NCBI | ||
<!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]--> | <!--*[http://www.ncbi.nlm.nih.gov/nuccore/?accessNr? '''GenBank''': ?title?]--> | ||
<!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]--> | <!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]--> | ||
Latest revision as of 10:30, 24 October 2012
CaMXMT1 expression cassette for yeast
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1433
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 205
Composite part of BBa_K319003, BBa_K801071 and BBa_K801012
Keywords:
caffeine, TEF1, expression cassette
Abbreviations:
- CaMXMT1 = coffea arabica 7-methylxanthine N-methyltransferase 1
Design Notes
Related BioBrick: BBa_K801071
Quality control measures:
- Test digest has been performed with use of Xba1 and Pst1 (see mainpage)
Backbone:
- Backbone name: pSB1C3'
- Resistance: Cp
Protein coding:
- Protein: CaMXMT1-Strep-tag
Enzymatic activity:
- EC-number [http://enzyme.expasy.org/EC/2.1.1.160 2.1.1.160]
Cytotoxicity:
This Biobrick is one part of the pathway, which leads to caffeine (the BioBricks BBa_801073 and BBa_801075 are further needed). We investigated the toxicity of caffeine and found out, that the growth of yeast strains was inhibited at concentrations of 10 mM. Caffeine with a concentration of 100 mM even showed a lethal effect on yeast cells. Lower concentrations of caffeine (range of micro molar) did not show any growth inhibition or effect.
Safety notes:
Intellectual property:
Corresponding part author/authors:
Source
Source:
- Preexisting BioBrick BBa_K801071
Organism:
- Genesequence derived from Coffea arabica
- codonoptimized for Saccharomyces cerevisiae
- designed for Saccharomyces cerevisiae
References
Literature references:
- [http://www.ncbi.nlm.nih.gov/pubmed/12746542 Pubmed: Uefuji H., Ogita S., Yamaguchi Y., Koizumi N. and Sano H., 2003: Molecular cloning and functional characterization of three distinct n-methyltransferases involved in the caffeine biosynthetic pathway in coffee plants.]
- [http://www.ncbi.nlm.nih.gov/pubmed/16247553 Pubmed: Uefuji H., Ogita S., Yamaguchi Y., Koizumi N. and Sano H., 2005: Caffeine production in tobacco plants by simultaneous expression of three coffee N-methyltrasferases and its potential as a pest repellant.]
- [http://www.ncbi.nlm.nih.gov/pubmed/16925551 Pubmed: Kuranda K, Leberre V, Sokol S, Palamarczyk G, François J., 2006: Investigating the caffeine effects in the yeast Saccharomyces cerevisiae brings new insights into the connection between TOR, PKC and Ras/cAMP signalling pathways.]
Database references:
- NCBI