Difference between revisions of "Part:BBa I721001:Experience"
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We grew liquid cultures for 24 hours with varying concentrations of Lead Acetate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). The results were measured in fluorescence per OD 600. As shown with the graph below, our results were inconsistent. We did not incorporate a lead binding protein into our design. Also, we noticed a precipitate starting at the .5 mM concentration. We feel that the precipitate or the lead's toxicity interfered with our results. | We grew liquid cultures for 24 hours with varying concentrations of Lead Acetate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). The results were measured in fluorescence per OD 600. As shown with the graph below, our results were inconsistent. We did not incorporate a lead binding protein into our design. Also, we noticed a precipitate starting at the .5 mM concentration. We feel that the precipitate or the lead's toxicity interfered with our results. | ||
Latest revision as of 23:09, 13 October 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_I721001
User Reviews
UNIQ87e96758fae2b472-partinfo-00000000-QINU UNIQ87e96758fae2b472-partinfo-00000001-QINU
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2012 Gaston Day School iGEM |
We grew liquid cultures for 24 hours with varying concentrations of Lead Acetate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). The results were measured in fluorescence per OD 600. As shown with the graph below, our results were inconsistent. We did not incorporate a lead binding protein into our design. Also, we noticed a precipitate starting at the .5 mM concentration. We feel that the precipitate or the lead's toxicity interfered with our results.
 UNIQ87e96758fae2b472-partinfo-00000003-QINU |