Difference between revisions of "Part:BBa K732002"

Latest revision as of 12:03, 1 October 2013

synCG-β + Promoter + RBS

Human chorionic gonadotropin (hCG) is a hormone produced during pregnancy. Its beta subunit (hCG-β) contains epitopes recognized by pregnancy tests. The full hCG dimer is also used as an ovulation inducer and can stimulate the production of testosterone in men.

This sequence could ideally be used to express hCG for any downstream uses, and also as an output signal since it could trigger pregnancy tests at low concentrations when properly folded.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 224
Illegal NgoMIV site found at 268
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K732002 short</partinfo>
-This construct contains a strong constitutive promoter (J23119) as well as an RBS (B0034) upstream of synCG-&#946; (K732001), and should strongly express a synthetic version of the beta subunit of human chorionic gonadotropin.
+Human chorionic gonadotropin (hCG) is a hormone produced during pregnancy. Its beta subunit (hCG-β) contains epitopes recognized by pregnancy tests. The full hCG dimer is also used as an ovulation inducer and can stimulate the production of testosterone in men.
-One application of this component is detection by pregnancy test.
+This sequence could ideally be used to express hCG for any downstream uses, and also as an output signal since it could trigger pregnancy tests at low concentrations when properly folded.
-'''Proof-of-principle'''
-When heterogeneous mixture of ''E. coli'' expressing BBa_K732001 and BBa_K732002 was lysed by either sonication or T7 infection, re-folded using a literature-based protocol, and applied to NewChoice pregnancy tests (purchased from Dollar Tree), positive results were visible.
-Note that (a) there was some coloration around the positive band, and (b) the pregnancy tests were left overnight for bands to become visible. We hypothesize that the delayed read-time is necessary because the folding solution interferes with band visibility.
-[[Image:Virginia 2012 Oct_2_Preg_Test_Results.jpg]]