Difference between revisions of "Part:BBa K945000:Experience"

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===Characterization by Tec-Monterrey EKAM iGEM 2012===
 
===Characterization by Tec-Monterrey EKAM iGEM 2012===
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The functionality of this promoter was tested by transforming ''Pichia pastoris'' with a construct containing reporter <partinfo>BBa_K945004</partinfo> under the control of this promoter. The culture was induced with methanol at a 1% concentration visualized using fluorescence microscopy.
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The functionality of this promoter was tested by transforming ''Pichia pastoris'' with a construct containing reporter <partinfo>BBa_K945004</partinfo> under the control of this promoter. The culture was induced with methanol at a 1% concentration and visualized using fluorescence microscopy.
  
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[[Image:VA.JPG | frame | center | '''Control''': ''Pichia pastoris'' visualized under the microscope.]]
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[[Image:VA.JPG | thumb | left | '''Control''': ''Pichia pastoris'' visualized under the microscope.]]
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[[Image:VB.JPG | thumb | left |'''Control''': ''Pichia pastoris'' visualized with fluorescence microscopy (Zeiss filter set 18).]]
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[[Image:G1A.png | thumb | left | Transformant visualized under the microscope.]]
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[[Image:G1B.png | thumb | left | Transformant visualized with fluorescence microscopy (Zeiss filter set 18).]]
  
 
===Applications of BBa_K945000===
 
===Applications of BBa_K945000===

Latest revision as of 10:45, 6 October 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Characterization by Tec-Monterrey EKAM iGEM 2012

The functionality of this promoter was tested by transforming Pichia pastoris with a construct containing reporter BBa_K945004 under the control of this promoter. The culture was induced with methanol at a 1% concentration and visualized using fluorescence microscopy.

Control: Pichia pastoris visualized under the microscope.
Control: Pichia pastoris visualized with fluorescence microscopy (Zeiss filter set 18).
Transformant visualized under the microscope.
Transformant visualized with fluorescence microscopy (Zeiss filter set 18).

Applications of BBa_K945000

User Reviews

UNIQa3cabfe1193facca-partinfo-00000001-QINU UNIQa3cabfe1193facca-partinfo-00000002-QINU