Difference between revisions of "Part:BBa K850001:Experience"
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| − | BBa_K850001 was excised from the Biobrick vector plasmid using Xba1 and Spe1 and cloned into the expression vector pUC19. Cell lysates from transformed and untransformed cells were compared by electrophoresis on a denaturing 12% polyacrylamide gel. Cells containing the BBa_K850001 insert in the proper orientation expressed a protein of about 26kD. The predicted molecular weight of NAG1 is 27.5 kD, strongly suggesting that the correct protein is being expressed. | + | BBa_K850001 was excised from the Biobrick vector plasmid using Xba1 and Spe1 and cloned into the expression vector pUC19. Cell lysates from transformed and untransformed cells were compared by electrophoresis on a denaturing 12% polyacrylamide gel. Cells containing the BBa_K850001 insert in the proper orientation (starred lanes) expressed a protein of about 26kD. The predicted molecular weight of NAG1 is 27.5 kD, strongly suggesting that the correct protein is being expressed. |
[[Image:Nag1.jpg]] | [[Image:Nag1.jpg]] | ||
Latest revision as of 03:31, 4 October 2012
BBa_K850001 was excised from the Biobrick vector plasmid using Xba1 and Spe1 and cloned into the expression vector pUC19. Cell lysates from transformed and untransformed cells were compared by electrophoresis on a denaturing 12% polyacrylamide gel. Cells containing the BBa_K850001 insert in the proper orientation (starred lanes) expressed a protein of about 26kD. The predicted molecular weight of NAG1 is 27.5 kD, strongly suggesting that the correct protein is being expressed.
Applications of BBa_K850001
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