Difference between revisions of "Part:BBa K902056:Design"
(→Design Notes) |
|||
| Line 10: | Line 10: | ||
===Source=== | ===Source=== | ||
| − | Amplified from a <i>Rhodococcus</i> environmental isolate via PCR. | + | Amplified from a <i>Rhodococcus</i> environmental isolate via PCR. This part was constructed using the basic biobrick assembly method. |
===References=== | ===References=== | ||
Xi L, Squires CH, Monticello DJ, Childs JD. A flavin reductase stimulates DszA and DszC proteins of Rhodococcus erythropolis IGTS8 in vitro. Biochem Biophys Res Commun. 1997 Jan 3;230(1):73-5. | Xi L, Squires CH, Monticello DJ, Childs JD. A flavin reductase stimulates DszA and DszC proteins of Rhodococcus erythropolis IGTS8 in vitro. Biochem Biophys Res Commun. 1997 Jan 3;230(1):73-5. | ||
Latest revision as of 02:33, 27 October 2012
dszC behind an RBS (B0034)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 96
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 900
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1050
Design Notes
Mutagenesis- See DszC page
Source
Amplified from a Rhodococcus environmental isolate via PCR. This part was constructed using the basic biobrick assembly method.
References
Xi L, Squires CH, Monticello DJ, Childs JD. A flavin reductase stimulates DszA and DszC proteins of Rhodococcus erythropolis IGTS8 in vitro. Biochem Biophys Res Commun. 1997 Jan 3;230(1):73-5.