Difference between revisions of "Part:BBa K902053:Design"

(→‎References)
 
Line 12: Line 12:
 
===Source===
 
===Source===
  
−
DszB amplified from an environmental isolate of Rhodococcus that is an active desulfurizer, promotor from registry.
+
DszB amplified from an environmental isolate of Rhodococcus that is an active desulfurizer, promotor from registry. This part was constructed using the basic biobrick assembly method.
  
 
===References===
 
===References===
 
Oshiro T, Ohkita R, Takikawa T, Manabe M, Lee WC, Tanokura M, Izumi Y. Improvement of 2'-hydroxybiphenyl-2-sulfinate desulfinase, an enzyme involved in the dibenzothiophene desulfurization pathway, from Rhodococcus erythropolis KA2-5-1 by site-directed mutagenesis. Biosci Biotechnol Biochem. 2007 Nov.; 71(11):2815-21
 
Oshiro T, Ohkita R, Takikawa T, Manabe M, Lee WC, Tanokura M, Izumi Y. Improvement of 2'-hydroxybiphenyl-2-sulfinate desulfinase, an enzyme involved in the dibenzothiophene desulfurization pathway, from Rhodococcus erythropolis KA2-5-1 by site-directed mutagenesis. Biosci Biotechnol Biochem. 2007 Nov.; 71(11):2815-21

Latest revision as of 02:53, 27 October 2012

Constitutive DszB generator (J13002-DszB)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 506
    Illegal XhoI site found at 498
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 213
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

See DszB mutations


Source

DszB amplified from an environmental isolate of Rhodococcus that is an active desulfurizer, promotor from registry. This part was constructed using the basic biobrick assembly method.

References

Oshiro T, Ohkita R, Takikawa T, Manabe M, Lee WC, Tanokura M, Izumi Y. Improvement of 2'-hydroxybiphenyl-2-sulfinate desulfinase, an enzyme involved in the dibenzothiophene desulfurization pathway, from Rhodococcus erythropolis KA2-5-1 by site-directed mutagenesis. Biosci Biotechnol Biochem. 2007 Nov.; 71(11):2815-21