Difference between revisions of "Part:BBa K900001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This version of PAGFP contains the A206K mutation, which should disrupt fluorescent protein dimerization even at high concentrations. Restriction sites were removed to be compatible with the 2012 Berkeley iGEM team's Golden Gate cloning scheme. | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | Cloned off of Addgene plasmid [http://www.addgene.org/11911/ 11911]. Previously engineered version of GFP from Patterson and Lippincott-Schwartz, 2002. | |
===References=== | ===References=== | ||
Patterson, G. H. & Lippincott-Schwartz, J. A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells. Science 297, 1873–1877 (2002). | Patterson, G. H. & Lippincott-Schwartz, J. A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells. Science 297, 1873–1877 (2002). | ||
Latest revision as of 14:54, 4 October 2012
PAGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This version of PAGFP contains the A206K mutation, which should disrupt fluorescent protein dimerization even at high concentrations. Restriction sites were removed to be compatible with the 2012 Berkeley iGEM team's Golden Gate cloning scheme.
Source
Cloned off of Addgene plasmid [http://www.addgene.org/11911/ 11911]. Previously engineered version of GFP from Patterson and Lippincott-Schwartz, 2002.
References
Patterson, G. H. & Lippincott-Schwartz, J. A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells. Science 297, 1873–1877 (2002).