Difference between revisions of "Part:BBa K842004"
 
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Cloning and Uses:
 
Cloning and Uses:
 
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3.  It is to be used in conjunction with the entire flagella synthesis genetic pathway to reproduce a complete flagella apparatus.
 
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3.  It is to be used in conjunction with the entire flagella synthesis genetic pathway to reproduce a complete flagella apparatus.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 01:08, 4 October 2012

fliC

USC flic.gif

Transcription of fliC generates the flagellin protein, which is then exported to form the flagella filament. This structure is the backbone of the flagella.

Used for reconstitution of flagella apparatus in E. coli B strains.

Cloning and Uses: This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used in conjunction with the entire flagella synthesis genetic pathway to reproduce a complete flagella apparatus.














Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 547
    Illegal PstI site found at 872
    Illegal PstI site found at 919
    Illegal PstI site found at 1486
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 547
    Illegal PstI site found at 872
    Illegal PstI site found at 919
    Illegal PstI site found at 1486
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1224
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 547
    Illegal PstI site found at 872
    Illegal PstI site found at 919
    Illegal PstI site found at 1486
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 547
    Illegal PstI site found at 872
    Illegal PstI site found at 919
    Illegal PstI site found at 1486
    Illegal AgeI site found at 301
    Illegal AgeI site found at 709
  • 1000
    COMPATIBLE WITH RFC[1000]