Difference between revisions of "Part:BBa K892009"

(→‎Usage and Biology)
(→‎Usage and Biology)
 
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===Usage and Biology===
 
===Usage and Biology===
 
This part was characterized by [http://2012.igem.org/Team:Washington Washington 2012] using the turbidostat. MG1655 cells were cotransformed with fucO and aldA, the assay is described on the [http://2012.igem.org/Team:Washington/Protocols/EG_Assay wiki] and the data is also presented on the [http://2012.igem.org/Team:Washington/Plastics#Results wiki].
 
This part was characterized by [http://2012.igem.org/Team:Washington Washington 2012] using the turbidostat. MG1655 cells were cotransformed with fucO and aldA, the assay is described on the [http://2012.igem.org/Team:Washington/Protocols/EG_Assay wiki] and the data is also presented on the [http://2012.igem.org/Team:Washington/Plastics#Results wiki].
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[[Image:Washington_Plastic_Overview.png|border|440px|center|thumb|The fucO aldA protein pathway as part of the plastic degradation project]]
+
[[Image:Washington_Plastic_Overview.png|border|900px|center|thumb|The fucO aldA protein pathway as part of the plastic degradation project]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 00:32, 4 October 2012

fucO

The gene fucO, which codes for glycolaldehyde reductase, is one of the genes required for E. coli to utilize ethylene glycol as a food source. The coding sequence is put behind a strong biofab promoter and RBS. This part should be used with aldA for the desired effect.

Usage and Biology

This part was characterized by [http://2012.igem.org/Team:Washington Washington 2012] using the turbidostat. MG1655 cells were cotransformed with fucO and aldA, the assay is described on the [http://2012.igem.org/Team:Washington/Protocols/EG_Assay wiki] and the data is also presented on the [http://2012.igem.org/Team:Washington/Plastics#Results wiki].

The fucO aldA protein pathway as part of the plastic degradation project

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 251
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 251
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 251
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 251
    Illegal AgeI site found at 967
    Illegal AgeI site found at 1168
  • 1000
    COMPATIBLE WITH RFC[1000]