Difference between revisions of "Part:BBa K892009"
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The gene fucO, which codes for glycolaldehyde reductase, is one of the genes required for <i>E. coli</i> to utilize ethylene glycol as a food source. The coding sequence is put behind a strong biofab promoter and RBS. This part should be used with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K892010 aldA] for the desired effect.
 
The gene fucO, which codes for glycolaldehyde reductase, is one of the genes required for <i>E. coli</i> to utilize ethylene glycol as a food source. The coding sequence is put behind a strong biofab promoter and RBS. This part should be used with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K892010 aldA] for the desired effect.
  
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===Usage and Biology===
 
===Usage and Biology===
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This part was characterized by [http://2012.igem.org/Team:Washington Washington 2012] using the turbidostat. MG1655 cells were cotransformed with fucO and aldA, the assay is described on the [http://2012.igem.org/Team:Washington/Protocols/EG_Assay wiki] and some of the data is shown below.
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This part was characterized by [http://2012.igem.org/Team:Washington Washington 2012] using the turbidostat. MG1655 cells were cotransformed with fucO and aldA, the assay is described on the [http://2012.igem.org/Team:Washington/Protocols/EG_Assay wiki] and the data is also presented on the [http://2012.igem.org/Team:Washington/Plastics#Results wiki].
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[[Image:Washington_Plastic_Overview.png|border|900px|center|thumb|The fucO aldA protein pathway as part of the plastic degradation project]]
  
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[[Image:Washington_Turbidostat_Pump.png|border|450px|left|thumb|M9 30 mM ethylene glycol media injected into the culture vessel every minute to maintain the arbitrary optical density of 0.3.]]
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===Usage and Biology===
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[[Image:Washington_Turbidostat_OD.png|border|450px|right|thumb|Optical density measurements of <i>E. coli</i> transformed with fucO pGA3K3 + aldA pGA1C3 within the turbidostat growing on M9 30 mM ethylene glycol media.]]
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Latest revision as of 00:32, 4 October 2012

fucO

The gene fucO, which codes for glycolaldehyde reductase, is one of the genes required for E. coli to utilize ethylene glycol as a food source. The coding sequence is put behind a strong biofab promoter and RBS. This part should be used with aldA for the desired effect.

Usage and Biology

This part was characterized by [http://2012.igem.org/Team:Washington Washington 2012] using the turbidostat. MG1655 cells were cotransformed with fucO and aldA, the assay is described on the [http://2012.igem.org/Team:Washington/Protocols/EG_Assay wiki] and the data is also presented on the [http://2012.igem.org/Team:Washington/Plastics#Results wiki].

The fucO aldA protein pathway as part of the plastic degradation project

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 251
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 251
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 251
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 251
    Illegal AgeI site found at 967
    Illegal AgeI site found at 1168
  • 1000
    COMPATIBLE WITH RFC[1000]