Difference between revisions of "Part:BBa K726016"

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===Usage and Biology===
 
===Usage and Biology===
 
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<regulartext1> A device was constructed that contained the three enzymes VioA, VioB, and VioE which were sufficient to produce a green pigment (prodeoxyviolacein) from tryptophan. <br>
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<regulartext1> A device was constructed that contained the three enzymes VioA, VioB, and VioE. <br>
  
 
<img align="center" style="margin-bottom:0px; width: 500px; margin-top:20px; padding:2; margin-left:55px;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/violacein/VioABE2.jpg"> <br>
 
<img align="center" style="margin-bottom:0px; width: 500px; margin-top:20px; padding:2; margin-left:55px;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/violacein/VioABE2.jpg"> <br>
  
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<img align="center" style="margin-bottom:0px; width: 500px; margin-top:20px; padding:2; margin-left:55px;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/violacein/IMG_4879.jpg"> <br>
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<regulartext> As can be seen in the images below, this three enzyme operon was sufficient to produce a green pigment (prodeoxyviolacein) from tryptophan present in the culture media. Samples were grown in LB media with or without IPTG
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<img align="center" style="margin-bottom:0px; width: 300px; margin-top:20px; padding:2; margin-left:55px;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/violacein/IMG_4879.jpg"> <br>
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<img align="center" style="margin-bottom:0px; width: 200px; margin-top:20px; padding:2; margin-left:55px;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/violacein/VioABE_Culture.jpg"> <br>
  
 
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Latest revision as of 20:50, 3 October 2012

T7Prom+HisTag+VioABE

The first three genes in the violacein operon, under the control of a T7 promoter with a lac operator and ribosome binding site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1147
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 123
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3929
    Illegal AgeI site found at 4125
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

A device was constructed that contained the three enzymes VioA, VioB, and VioE.

As can be seen in the images below, this three enzyme operon was sufficient to produce a green pigment (prodeoxyviolacein) from tryptophan present in the culture media. Samples were grown in LB media with or without IPTG .