Difference between revisions of "Part:BBa K842015"

(New page: Description and Function T7 serves at as a promoter that is stimulated by IPTG. RBS is a sequence in DNA located upstream of the start codon. It affects the rate at which the open reading ...)
 
 
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Description and Function
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T7 serves at as a promoter that is stimulated by IPTG. RBS is a sequence in DNA located upstream of the start codon. It affects the rate at which the open reading frame is translated. CheY is regulatory protein that revereses the direction of flagella rotation from conter-clockwise to clockwise. When the methyl-accepting chemotaxis protein (MCP) is not bound to any attractant, cheA is activated and told to self-phosphorylate itself by hydrolyzing ATP. Once the cheA is phosphorylated, it transfers its phosphate group to cheY, therefore activating it. CheY is then moves to the cytoplasmic side of the flagella apparatus and binds to it. When flagella are not bound to cheY, it rotates in a counter-clockwise direction, which allows the bacteria to move in a “forward” direction. Flagella that are bound to phosphorylated cheY switches its rotation to clockwise, which induces tumbling.
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<partinfo>BBa_K842015 short</partinfo>
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[[Image:USC_T7_RBS_cheY.gif|300px|right]]
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T7 serves as a promoter that is stimulated by IPTG. RBS is a sequence in DNA located upstream of the start codon. It affects the rate at which the open reading frame is translated. CheY is regulatory protein that reverses the direction of flagella rotation from counter-clockwise to clockwise. When the methyl-accepting chemotaxis protein (MCP) is not bound to any attractant, cheA is activated and told to self-phosphorylate itself by hydrolyzing ATP. Once the cheA is phosphorylated, it transfers its phosphate group to cheY, therefore activating it. CheY then moves to the cytoplasmic side of the flagella apparatus and binds to it. When flagella are not bound to cheY, it rotates in a counter-clockwise direction, which allows the bacteria to move in a “forward” direction. Flagella that are bound to phosphorylated cheY switches its rotation to clockwise, which induces tumbling.
 
Used for researcher control of signaling mechanisms that influence flagella function.
 
Used for researcher control of signaling mechanisms that influence flagella function.
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https://parts.igem.org/wiki/index.php?title=Part:BBa_K842015
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Cloning and Uses
 
Cloning and Uses
 
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used with other chemotaxis genes to form a complete regulatory pathway that controls the response system in MCP and the direction of rotation in flagella.
 
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used with other chemotaxis genes to form a complete regulatory pathway that controls the response system in MCP and the direction of rotation in flagella.
  
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<p><strong>Overexpression of <em>cheY</em> induces tumbling in all cells</strong></p>
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Sources
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<p>http://uscigem2012.files.wordpress.com/2012/10/cheytumbling.gif</p>
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Chemotaxis protein CheY – Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720). (n.d.). UniProt. Retrieved October 1, 2012, from http://www.uniprot.org/uniprot/P0A2D5
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K8420015 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K842015 SequenceAndFeatures</partinfo>
  
  

Latest revision as of 15:56, 10 October 2012

T7 RBS cheY

USC T7 RBS cheY.gif

T7 serves as a promoter that is stimulated by IPTG. RBS is a sequence in DNA located upstream of the start codon. It affects the rate at which the open reading frame is translated. CheY is regulatory protein that reverses the direction of flagella rotation from counter-clockwise to clockwise. When the methyl-accepting chemotaxis protein (MCP) is not bound to any attractant, cheA is activated and told to self-phosphorylate itself by hydrolyzing ATP. Once the cheA is phosphorylated, it transfers its phosphate group to cheY, therefore activating it. CheY then moves to the cytoplasmic side of the flagella apparatus and binds to it. When flagella are not bound to cheY, it rotates in a counter-clockwise direction, which allows the bacteria to move in a “forward” direction. Flagella that are bound to phosphorylated cheY switches its rotation to clockwise, which induces tumbling. Used for researcher control of signaling mechanisms that influence flagella function.

Cloning and Uses This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used with other chemotaxis genes to form a complete regulatory pathway that controls the response system in MCP and the direction of rotation in flagella.

Overexpression of cheY induces tumbling in all cells

http://uscigem2012.files.wordpress.com/2012/10/cheytumbling.gif

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]