Difference between revisions of "Part:BBa K902018"

Latest revision as of 21:52, 3 October 2012

MgtAp-mgtArb-S7

This circuit was built to characterize the mgtAp-mgtArb and test how well it would function as a killswitch. S7 micrococcal nuclease was constructed with the regulatory elements and we took readings for OD600 as well as did a CFU testing. This system is repressed in the presence of magnesium and is turned on in the absence of magensium. E. coli uses the mgtA promoter and riboswitch combination to maintain magnesium homeostasis in the cell. This killswitch is activated in the lack of magensium. See experience page for more details.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 MgtAp-mgtArb-S7
+<html><p> This circuit was built to characterize the mgtAp-mgtArb and test how well it would function as a killswitch. S7 micrococcal nuclease was constructed with the regulatory elements and we took readings for OD600 as well as did a CFU testing. This system is repressed in the presence of magnesium and is turned on in the absence of magensium. <i>E. coli</i> uses the mgtA promoter and riboswitch combination to maintain magnesium homeostasis in the cell. This killswitch is activated in the lack of magensium. See experience page for more details.</p> </html>
-<html><p>This is the magnesium circuit with the S7 micrococal nuclease. An assay was done measure optical density of E. coli with different concentrations of magnesium.</p></html>[[Image:24 hour assay with mgtap-rb-S7.png|750px|center]]<html> <p> The drop in OD in the mgtAp-mgtArb-S7 circuit compared to the negative control indicates that the S7 is functional. It also demonstrates that 10mM MgCl2 is not enough.</html>
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