Difference between revisions of "Part:BBa K801063:Design"

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'''Backbone:'''<br>
 
'''Backbone:'''<br>
−
*Backbone name: pSB1C3'
+
* Backbone name: pSB1C3
−
<!--*Resistance: Amp/Cp/Kan/Tet-->
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* Resistance: Cp
−
<!--*Copynumber: low/medium/high-->
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* Copynumber: high
  
 
'''Protein coding:'''<br>
 
'''Protein coding:'''<br>
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<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
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* BioBrick encodes (+)-limonene synthase
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<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
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* UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]
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<!--*The protein encoded is posttranslationally modified by ???.-->
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<!--none/not known/cytotoxic for ''organism name''-->
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<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
+
  
 
'''Enzymatic activity:'''
 
'''Enzymatic activity:'''
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EC-number  4.2.3.20
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* E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]
  
 
'''Cytotoxicity:'''<br>
 
'''Cytotoxicity:'''<br>
−
<!--none/not known/cytotoxic for ''organism name''-->
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cytotoxic for ''S.cervisiae'' growth inhibition at 1 mM, lethal effect at 100 mM
  
 
'''Safety notes:'''<br>
 
'''Safety notes:'''<br>

Latest revision as of 11:28, 16 October 2012

(+)-limonene synthase 1 expression cassette for yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2127
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 211
    Illegal SapI.rc site found at 561


This is a composite part consisting of the yeast TEF1 promoter (BBa_K319003), the Citrus limon (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast TEF1 terminator (BBa_K801011).


Keywords: limonen


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

  • Backbone name: pSB1C3
  • Resistance: Cp
  • Copynumber: high

Protein coding:

  • BioBrick encodes (+)-limonene synthase
  • UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]

Enzymatic activity:

  • E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]

Cytotoxicity:
cytotoxic for S.cervisiae growth inhibition at 1 mM, lethal effect at 100 mM

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

  • BioBrick: BBa_I742111


Organism:

  • Gene sequence derived from Citrus limon

References

Literature references:

  • http://www.ncbi.nlm.nih.gov/pubmed/12084056 Lücker et al., 2002 Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A. (2002). Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71.

Database references: