Difference between revisions of "Part:BBa K842009"

(New page: Description and Function CheY is regulatory protein that revereses the direction of flagella rotation from conter-clockwise to clockwise. When the methyl-accepting chemotaxis protein (MCP)...)
 
 
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Description and Function
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CheY is regulatory protein that revereses the direction of flagella rotation from conter-clockwise to clockwise. When the methyl-accepting chemotaxis protein (MCP) is not bound to any attractant, cheA is activated and told to self-phosphorylate itself by hydrolyzing ATP. Once the cheA is phosphorylated, it transfers its phosphate group to cheY, therefore activating it. CheY is then moves to the cytoplasmic side of the flagella apparatus and binds to it. When flagella is not bound to cheY, it rotates in a counter-clockwise direction, which allows the bacteria to move in a “forward” direction. Flagella that is bound to phosphorylated cheY switches its rotation to clockwise, which induces tumbling.
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<partinfo>BBa_K842009 short</partinfo>
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[[Image:USC_chey.gif|300px|right]]
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CheY is regulatory protein that reverses the direction of flagella rotation from counter-clockwise to clockwise. When the methyl-accepting chemotaxis protein (MCP) is not bound to any attractant, cheA is activated and told to self-phosphorylate itself by hydrolyzing ATP. Once the cheA is phosphorylated, it transfers its phosphate group to cheY, therefore activating it. CheY then moves to the cytoplasmic side of the flagella apparatus and binds to it. When flagella is not bound to cheY, it rotates in a counter-clockwise direction, which allows the bacteria to move in a “forward” direction. Flagella that is bound to phosphorylated cheY switches its rotation to clockwise, which induces tumbling.
 
Used for researcher control of signaling mechanisms that influence flagella function.
 
Used for researcher control of signaling mechanisms that influence flagella function.
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https://parts.igem.org/wiki/index.php?title=Part:BBa_K842009
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Cloning and Uses
 
Cloning and Uses
 
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used with other chemotaxis genes to form a complete regulatory pathway that controls the response system in MCP and the direction of rotation in flagella.
 
This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used with other chemotaxis genes to form a complete regulatory pathway that controls the response system in MCP and the direction of rotation in flagella.
  
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Sources
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Chemotaxis protein CheY – Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720). (n.d.). UniProt. Retrieved October 1, 2012, from http://www.uniprot.org/uniprot/P0A2D5
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K842009 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K842009 parameters</partinfo>
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Latest revision as of 01:30, 4 October 2012

cheY

USC chey.gif

CheY is regulatory protein that reverses the direction of flagella rotation from counter-clockwise to clockwise. When the methyl-accepting chemotaxis protein (MCP) is not bound to any attractant, cheA is activated and told to self-phosphorylate itself by hydrolyzing ATP. Once the cheA is phosphorylated, it transfers its phosphate group to cheY, therefore activating it. CheY then moves to the cytoplasmic side of the flagella apparatus and binds to it. When flagella is not bound to cheY, it rotates in a counter-clockwise direction, which allows the bacteria to move in a “forward” direction. Flagella that is bound to phosphorylated cheY switches its rotation to clockwise, which induces tumbling. Used for researcher control of signaling mechanisms that influence flagella function.

Cloning and Uses This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used with other chemotaxis genes to form a complete regulatory pathway that controls the response system in MCP and the direction of rotation in flagella.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]