Difference between revisions of "Part:BBa K783083"

 
 
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<partinfo>BBa_K783083 short</partinfo>
 
<partinfo>BBa_K783083 short</partinfo>
  
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mmoR is a sigma-54 regulatory activator amplified from Methylosinus trichosporium OB3b and positively regulates pMmoR (BBa_K783066). Copper represses that activity. This has two MoClo fusion sites flanking the part.
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<i>mmoR</i> is a sigma-54 regulatory activator amplified from <i>Methylosinus trichosporium</i> OB3b and positively regulates pMmoR (BBa_K783066). Copper represses that activity.  
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This has two MoClo fusion sites flanking the part: 5' C [part] D 3'
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The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
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Note: This gene has three PstI cut sites, so this cannot be built using standard BioBrick protocols.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:11, 7 October 2012

mmoR from M. trichosporium OB3b MoClo format

mmoR is a sigma-54 regulatory activator amplified from Methylosinus trichosporium OB3b and positively regulates pMmoR (BBa_K783066). Copper represses that activity.

This has two MoClo fusion sites flanking the part: 5' C [part] D 3'

The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Note: This gene has three PstI cut sites, so this cannot be built using standard BioBrick protocols.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1130
    Illegal PstI site found at 1406
    Illegal PstI site found at 2039
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1130
    Illegal PstI site found at 1406
    Illegal PstI site found at 2039
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 35
    Illegal XhoI site found at 407
    Illegal XhoI site found at 1883
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1130
    Illegal PstI site found at 1406
    Illegal PstI site found at 2039
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1130
    Illegal PstI site found at 1406
    Illegal PstI site found at 2039
    Illegal NgoMIV site found at 80
    Illegal NgoMIV site found at 262
    Illegal NgoMIV site found at 508
    Illegal NgoMIV site found at 776
    Illegal NgoMIV site found at 860
    Illegal NgoMIV site found at 1197
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1103