Difference between revisions of "Part:BBa K892402"

Latest revision as of 16:25, 3 October 2012

HB36.5 F317S

A mutated HB36.5 flu binder aimed to increase binding to H2, a subtype of hemagglutinin on the surface of the Influenza virus. This mutant has a point mutation at residue 317 from Phenylalanine to Serine

Usage and Biology

This part was tested by the [http://2012.igem.org/Team:Washington 2012 University of Washington] iGEM team to bind to various subtypes of Hemagglutinin in Group 1.To test BBa _K892401, it was inserted into a protein expression vector, pETCON. HB36.5_F317H was then produced and transformed into yeast as described in the [http://2012.igem.org/Team:Washington/Protocols/Yeast 2012 Team's Yeast Transformation Protocol]. The binding protein was then tested for activity against H2. For a detailed description of the methodology, please see the [http://2012.igem.org/Team:Washington/Protocols/Display 2012 UW iGEM Yeast Surface Display protocol]. The resulting data is shown below.

HB36.5 and its variants tested against hemagglutinin subtype 2. HB36.5 is shown to have very little activity in binding H2.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 107
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 107
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 289
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 107
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 107
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ===Usage and Biology===
-This part was tested by the [http://2012.igem.org/Team:Washington 2012 University of Washington] iGEM team to bind to various subtypes of Hemagglutinin in Group 1.To test BBa _K892401, it was inserted into a protein expression vector, pETCON. HB36.5 was then produced and transformed into yeast as described in the [http://2012.igem.org/Team:Washington/Protocols/Yeast  2012 Team's Yeast Transformation Protocol]. The binding protein was then tested for activity against H2. For a detailed description of the methodology, please see the [http://2012.igem.org/Team:Washington/Protocols/Display 2012 UW iGEM Yeast Surface Display protocol]. The resulting data is shown below.
+This part was tested by the [http://2012.igem.org/Team:Washington 2012 University of Washington] iGEM team to bind to various subtypes of Hemagglutinin in Group 1.To test BBa _K892401, it was inserted into a protein expression vector, pETCON. HB36.5_F317H was then produced and transformed into yeast as described in the [http://2012.igem.org/Team:Washington/Protocols/Yeast  2012 Team's Yeast Transformation Protocol]. The binding protein was then tested for activity against H2. For a detailed description of the methodology, please see the [http://2012.igem.org/Team:Washington/Protocols/Display 2012 UW iGEM Yeast Surface Display protocol]. The resulting data is shown below.
 [[Image:UWashington FLU HB36 results graph.png|border|800px|center|thumb|HB36.5 and its variants tested against hemagglutinin subtype 2. HB36.5 is shown to have very little activity in binding H2.]]