Difference between revisions of "Part:BBa K801000:Design"
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'''Source:'''<br> | '''Source:'''<br> | ||
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<!--Thank you very much for using BBF RFC 82 to describe the present BioBrick. If you have any remarks or recommendations concerning this RFC we would highly appreciate to get your feedback on: []--> | <!--Thank you very much for using BBF RFC 82 to describe the present BioBrick. If you have any remarks or recommendations concerning this RFC we would highly appreciate to get your feedback on: []--> | ||
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===References=== | ===References=== | ||
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'''Literature references:'''<br> | '''Literature references:'''<br> | ||
| − | + | *[[http://www.ncbi.nlm.nih.gov/pubmed/6392852 West et al., 1984]] West, R. W. J., Yocum, R. R., and Ptashne, M. (1984). Saccharomyces cerevisiae GAL1-GAL10 Divergent Promoter Region: Location and Function of the Upstream Activator Sequence UASG. ''Mol. Cell. Biol.''4, 2467-2478. | |
| + | *[[http://www.ncbi.nlm.nih.gov/pubmed/3886158 Giniger et al., 1985]] Giniger, E., Barnum, S. M., and Ptashne, M. (1985). Specific DNA Binding of GAL4, a Positive Regulatory Protein of Yeast. ''Cell'' 40, 767-774. | ||
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'''Database references:'''<br> | '''Database references:'''<br> | ||
Latest revision as of 08:24, 15 October 2012
pTUM100 yeast shuttle vector based on pYES2
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 36
Illegal suffix found at 114 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 36
Illegal NheI site found at 3379
Illegal SpeI site found at 115
Illegal PstI site found at 129
Illegal NotI site found at 42
Illegal NotI site found at 122 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 36
Illegal XhoI site found at 63 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 36
Illegal suffix found at 115 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 36
Illegal suffix found at 105
Illegal XbaI site found at 51
Illegal NgoMIV site found at 69 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 79
Illegal BsaI.rc site found at 1536
Illegal BsaI.rc site found at 2482
Illegal SapI site found at 453
Illegal SapI site found at 2634
Keywords:
yeast shuttle vector, yeast, yeast vector, pTUM, pYES2
Abbreviations:
Design Notes
Related BioBrick:
Other versions:
- BBa_K801001: pTUM101 yeast shuttle vector with pTEF1 promoter
- BBa_K801002: pTUM102 yeast shuttle vector with pTEF2 promoter
- BBa_K801003: pTUM103 yeast shuttle vector with pADH1 promoter
- BBa_K801004: pTUM104 yeast shuttle vector with GAL1 promoter
Quality control measures:
- Part was totally sequenced
Backbone:
Protein coding:
Enzymatic activity:
Cytotoxicity:
Safety notes:
Intellectual property:
Corresponding part author/authors:
Source
Source:
Commercial system: pYES2 , Invitrogen
Organism:
References
Literature references:
- http://www.ncbi.nlm.nih.gov/pubmed/6392852 West et al., 1984 West, R. W. J., Yocum, R. R., and Ptashne, M. (1984). Saccharomyces cerevisiae GAL1-GAL10 Divergent Promoter Region: Location and Function of the Upstream Activator Sequence UASG. Mol. Cell. Biol.4, 2467-2478.
- http://www.ncbi.nlm.nih.gov/pubmed/3886158 Giniger et al., 1985 Giniger, E., Barnum, S. M., and Ptashne, M. (1985). Specific DNA Binding of GAL4, a Positive Regulatory Protein of Yeast. Cell 40, 767-774.
Database references: