Difference between revisions of "Part:BBa K801098:Design"

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(→‎Design Notes)
 
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Other versions: [https://parts.igem.org/Part:BBa_K801097 BBa_K801097: OMT1 + yeast consensus sequence]
 
Other versions: [https://parts.igem.org/Part:BBa_K801097 BBa_K801097: OMT1 + yeast consensus sequence]
  
−
Related BioBricks: [https://parts.igem.org/Part:BBa_K801090 BBa_K801090], [https://parts.igem.org/Part:BBa_K801091 BBa_K801091], [https://parts.igem.org/Part:BBa_K801092 BBa_K801092], [https://parts.igem.org/Part:BBa_K801093 BBa_K801093], [https://parts.igem.org/Part:BBa_K801094 BBa_K801094], [https://parts.igem.org/Part:BBa_K801095 BBa_K801095], [https://parts.igem.org/Part:BBa_K801096 BBa_K801096], [https://parts.igem.org/Part:BBa_K801097 BBa_K801097]  
+
Related BioBricks:  
 +
* [https://parts.igem.org/Part:BBa_K801090 BBa_K801090: phenylalanine ammonia lyase (PAL) + yeast consensus sequence]
 +
* [https://parts.igem.org/Part:BBa_K801091 BBa_K801091: phenylalanine ammonia lyase (PAL) coding region]
 +
* [https://parts.igem.org/Part:BBa_K801092 BBa_K801092: 4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence]
 +
* [https://parts.igem.org/Part:BBa_K801093 BBa_K801093: 4-coumarate--coenzyme A ligase (4CL) coding region]
 +
* [https://parts.igem.org/Part:BBa_K801094 BBa_K801094: Naringenin - chalcone synthase (CHS) + yeast consensus sequence]
 +
* [https://parts.igem.org/Part:BBa_K801095 BBa_K801095: Naringenin - chalcone synthase (CHS) coding region]
 +
* [https://parts.igem.org/Part:BBa_K801096 BBa_K801096: Aromatic prenyltransferase (APT) coding region]
 +
* [https://parts.igem.org/Part:BBa_K801097 BBa_K801097: Chalcone O-methyltransferase (OMT1) + yeast consensus sequence]  
  
 
'''Cloning details:'''<br>
 
'''Cloning details:'''<br>
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'''Backbone:'''<br>
 
'''Backbone:'''<br>
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Backbone name: pSB1C3
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*Backbone name: pSB1C3
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Resistance: Cp
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*Resistance: Cp
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<!--*Copynumber: low/medium/high-->
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*Copynumber: high
  
 
'''Protein coding:'''<br>
 
'''Protein coding:'''<br>
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Protein: O-methyltransferase
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*Protein: O-methyltransferase
 
<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
 
<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
 
<!--*The protein encoded is posttranslationally modified by ???.-->
 
<!--*The protein encoded is posttranslationally modified by ???.-->
−
<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
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*Tag: c-terminally fused Strep
  
 
'''Enzymatic activity:'''<br>
 
'''Enzymatic activity:'''<br>

Latest revision as of 12:21, 3 October 2012

O-methyltransferase 1 (OMT1) coding region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 479
    Illegal XhoI site found at 294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



http://www.ncbi.nlm.nih.gov/nucleotide/167613934/


Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, aromatic prenyltransferase


Abbreviations: OMT = O-methyltransferase

Design Notes

Related BioBrick:

Other versions: BBa_K801097: OMT1 + yeast consensus sequence

Related BioBricks:

Cloning details:
Designed in RFC10/RFC25

Quality control measures:
Part was totally sequenced

Backbone:

  • Backbone name: pSB1C3
  • Resistance: Cp
  • Copynumber: high

Protein coding:

  • Protein: O-methyltransferase
  • Tag: c-terminally fused Strep

Enzymatic activity:
EC-number: EC 2.1.1

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Plasmid with OMT provided by Jonathan E. Page, National Research Council Canada, Plant Biotechnology Institute, Canada


Organism:
Genesequence derived from Humulus lupulus

References

Literature references:
[http://www.ncbi.nlm.nih.gov/pubmed/18223037 Pubmed : Nagel, J., Culley, L.K., Lu, Y., Liu, E., Matthews, P.D., Stevens, J.F., Page, J.E., 2008. EST analysis of hop glandular trichomes identifies an O-methyltransferase that catalyzes the biosynthesis of xanthohumol. Plant Cell 20, 186–200]


Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/EU309725.1 GenBank: Humulus lupulus O-methyltransferase 1 (OMT1) mRNA, complete cds]

[http://www.uniprot.org/uniprot/B0ZB55 Uniprot: O-methyltransferase 1]