Difference between revisions of "Part:BBa K801094:Design"

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Other versions: [https://parts.igem.org/Part:BBa_K801095 BBa_K801095: CHS coding region ]
 
Other versions: [https://parts.igem.org/Part:BBa_K801095 BBa_K801095: CHS coding region ]
  
−
Related BioBricks: [https://parts.igem.org/Part:BBa_K801090 BBa_K801090], [https://parts.igem.org/Part:BBa_K801091 BBa_K801091], [https://parts.igem.org/Part:BBa_K801092 BBa_K801092], [https://parts.igem.org/Part:BBa_K801093 BBa_K801093], [https://parts.igem.org/Part:BBa_K801095 BBa_K801095], [https://parts.igem.org/Part:BBa_K801096 BBa_K801096], [https://parts.igem.org/Part:BBa_K801097 BBa_K801097], [https://parts.igem.org/Part:BBa_K801098 BBa_K801098]  
+
Related BioBricks:  
 +
* [https://parts.igem.org/Part:BBa_K801090 BBa_K801090: phenylalanine ammonia lyase (PAL) + yeast consensus sequence]
 +
* [https://parts.igem.org/Part:BBa_K801091 BBa_K801091: phenylalanine ammonia lyase (PAL) coding region]
 +
* [https://parts.igem.org/Part:BBa_K801092 BBa_K801092: 4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence]
 +
* [https://parts.igem.org/Part:BBa_K801093 BBa_K801093: 4-coumarate--coenzyme A ligase (4CL) coding region]
 +
* [https://parts.igem.org/Part:BBa_K801095 BBa_K801095: Naringenin - chalcone synthase (CHS) coding region]
 +
* [https://parts.igem.org/Part:BBa_K801096 BBa_K801096: Aromatic prenyltransferase (APT) coding region]
 +
* [https://parts.igem.org/Part:BBa_K801097 BBa_K801097: Chalcone O-methyltransferase (OMT1) + yeast consensus sequence]
 +
* [https://parts.igem.org/Part:BBa_K801098 BBa_K801098: O-methyltransferase 1 (OMT1) coding region]  
 +
 
  
 
'''Cloning details:'''<br>
 
'''Cloning details:'''<br>
−
Designed in RFC25
+
Designed in RFC10/RFC25
 
<!--*Mutation C889G to delete XbaI restriction site-->
 
<!--*Mutation C889G to delete XbaI restriction site-->
 
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
 
<!--*Truncation upstream/downstream compared to template, ?explanation?-->
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'''Backbone:'''<br>
 
'''Backbone:'''<br>
−
Backbone name: pSB1C3
+
*Backbone name: pSB1C3
−
Resistance: Cp
+
*Resistance: Cp
−
<!--*Copynumber: low/medium/high-->
+
*Copynumber: high
  
 
'''Protein coding:'''<br>
 
'''Protein coding:'''<br>
−
Protein: naringenin-chalcone synthase
+
*Protein: naringenin-chalcone synthase
 
<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
 
<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
 
<!--*The protein encoded is posttranslationally modified by ???.-->
 
<!--*The protein encoded is posttranslationally modified by ???.-->
−
<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
+
*Tag: c-terminally fused Strep
  
 
'''Enzymatic activity:'''<br>
 
'''Enzymatic activity:'''<br>

Latest revision as of 12:17, 3 October 2012

Naringenin - chalcone synthase (CHS) + yeast consensus sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 723
    Illegal BsaI.rc site found at 1144
    Illegal SapI.rc site found at 846



Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

http://www.ncbi.nlm.nih.gov/nucleotide/11096318/

PMID: 15932991


Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase, aromatic prenyltransferase


Abbreviations: CHS = naringenin-chalcone synthase

Design Notes

Related BioBrick:

Other versions: BBa_K801095: CHS coding region

Related BioBricks:


Cloning details:
Designed in RFC10/RFC25

Quality control measures:
Part was totally sequenced

Backbone:

  • Backbone name: pSB1C3
  • Resistance: Cp
  • Copynumber: high

Protein coding:

  • Protein: naringenin-chalcone synthase
  • Tag: c-terminally fused Strep

Enzymatic activity:
EC-number: 2.3.1.74

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA


Organism:
Genesequence derived from Hypericum androsaemum

References

Literature references:
[http://www.ncbi.nlm.nih.gov/pubmed/15932991 Pubmed : Jiang, H., Wood, K.V., Morgan, J.A., 2005. Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71, 2962–2969]


Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/AF315345.1 GenBank: Hypericum androsaemum chalcone synthase mRNA, complete cds]

[http://www.uniprot.org/uniprot/Q9FUB7 Uniprot: Chalcone synthase]