Difference between revisions of "Part:BBa K902070:Design"
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===Source=== | ===Source=== | ||
| − | + | RhaS was amplified from Top Ten E. coli with Kapa Hi-Fi polymerase. This part was then assembled with a strong RBS. | |
===References=== | ===References=== | ||
| + | Egan SM, Schleif RF, others. A regulatory cascade in the inductionof rhaBAD. J Mol Biol 1993;234(1):87-98. | ||
Latest revision as of 03:47, 27 October 2012
B0034-rhaS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 458
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 190
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The rhaS gene with a strong RBS.
Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki] for more design details.
Source
RhaS was amplified from Top Ten E. coli with Kapa Hi-Fi polymerase. This part was then assembled with a strong RBS.
References
Egan SM, Schleif RF, others. A regulatory cascade in the inductionof rhaBAD. J Mol Biol 1993;234(1):87-98.