Difference between revisions of "Part:BBa K902035"

Latest revision as of 21:07, 26 October 2012

AmdA Coding Sequence

AmdA is an amidase gene from Rhodococcus erythropolis that can remove amine and amide groups from carbon rings. It can potentially replace the function of the genes responsible for the lower half of the carbazole degradation pathway by cleaving the second C-N bond after the carA enzyme has converted carbazole into 2'-aminobiphenyl-2,3-diol. It can also remove the nitrogen from amides and replace it with a hydroxyl group, creating a carboxylic acid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 590
Illegal XhoI site found at 82
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 90
Illegal NgoMIV site found at 1295
Illegal NgoMIV site found at 1401
Illegal AgeI site found at 468
Illegal AgeI site found at 652
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 326
Illegal BsaI site found at 867

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 <partinfo>BBa_K902035 short</partinfo>
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-<i>AmdA</i> is a deaminase gene from <i>Rhodococcus erythropolis</i> that can remove amino groups from carbon rings. It can potentially replace the function of the genes responsible for the lower half of the carbazole degradation pathway by cleaving the second C-N bond after the <i>carA</i> enzyme has converted carbazole into 2'-aminobiphenyl-2,3-diol.
+<i>AmdA</i> is an amidase gene from <i>Rhodococcus erythropolis</i> that can remove amine and amide groups from carbon rings. It can potentially replace the function of the genes responsible for the lower half of the carbazole degradation pathway by cleaving the second C-N bond after the <i>carA</i> enzyme has converted carbazole into 2'-aminobiphenyl-2,3-diol. It can also remove the nitrogen from amides and replace it with a hydroxyl group, creating a carboxylic acid.
 </html>[[Image:amidase pathway kilbane calgary12.jpg|center]]