Difference between revisions of "Part:BBa K902034"
 
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The <i>carAd</i> gene in <i>Pseudomonas putida</i> codes for the ferredoxin reductase component of carbazole-1,9-dioxygenase. Together with <i>carAa</i> and <i>carAc</i> it forms the carbazole-1,9-dioxygenase (CARDO) enzyme which converts carbazole into 2'-aminobiphenyl-2,3-diol by cleaving one C-N bond in the original carbazole molecule.<br>
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The <i>carAd</i> gene in <i>Pseudomonas putida</i> codes for the ferredoxin reductase component of carbazole-1,9-dioxygenase. Together with <a href="https://parts.igem.org/Part:BBa_K902032"><i>carAa</i></a> and <a href="https://parts.igem.org/Part:BBa_K902033"><i>carAc</i></a> it forms the carbazole-1,9-dioxygenase (<i>carA</i>) enzyme which converts carbazole into 2'-aminobiphenyl-2,3-diol by cleaving one C-N bond in the original carbazole molecule.
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</html>[[File:CarA pathway Calgary12.jpg|thumb|420px|center|Action of the CarA enzyme complex.]]
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</html>[[Image:CarA pathway Calgary12.jpg|center]]
  
  

Latest revision as of 05:37, 3 October 2012

CarAd Coding Sequence

The carAd gene in Pseudomonas putida codes for the ferredoxin reductase component of carbazole-1,9-dioxygenase. Together with carAa and carAc it forms the carbazole-1,9-dioxygenase (carA) enzyme which converts carbazole into 2'-aminobiphenyl-2,3-diol by cleaving one C-N bond in the original carbazole molecule.
CarA pathway Calgary12.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 63
    Illegal SapI.rc site found at 51