Difference between revisions of "Part:BBa K323066:Experience"

Latest revision as of 05:11, 3 October 2012

We used this part in combination with parts BBa_K323132 and BBa_K323135 and showed that this DNA program leads to higher yields of violacein production. Check results.

Applications of BBa_K323066

Experience by 2012 iGEM NCTU_FORMOSA

The design of our biobricks(Part:BBa_K887000) has the same order of zinc finger as 2010 Slovenia iGEM team, so we decided to use the DNA program (Part:BBa_K323066)

However, after sequencing, we found that there is a deletion of a base pair in the zif268 biding site.

So, we designed a complementary primer to insert the lost base pair. After PCR of point mutation, we sequence it again.

And the outcome is below

As a result, we successfully correct the lost G base at the zif268 binding site.

More information, please refer to Part:BBa_K887011

User Reviews

UNIQf1d0d2e33cb6086b-partinfo-00000000-QINU UNIQf1d0d2e33cb6086b-partinfo-00000001-QINU

@@ Line 7: / Line 7: @@
 == '''Experience by 2012 iGEM NCTU_FORMOSA''' ==
-The design of our biobricks([[Part:K887000]]) has the same order of zinc finger as 2010 Slovenia iGEM team, so we decided to use the DNA program ([[Part:BBa_K323066]])
+The design of our biobricks([[Part:BBa_K887000]]) has the same order of zinc finger as 2010 Slovenia iGEM team, so we decided to use the DNA program ([[Part:BBa_K323066]])<br>
+However, after sequencing, we found that there is a deletion of a base pair in the zif268 biding site.
+[[image:DDpicture1.jpg|700px]]<br>
+So, we designed a complementary primer to insert the lost base pair. After PCR of point mutation, we sequence it again.<br>
+And the outcome is below
+[[image:DDpicture2.jpg|700px]]<br>
+As a result, we successfully correct the lost G base at the zif268 binding site.
+More information, please refer to [[Part:BBa_K887011]]
 ===User Reviews===
 <!-- DON'T DELETE --><partinfo>BBa_K323066 StartReviews</partinfo>