Difference between revisions of "Part:BBa K778002"
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fhlA-E363G has one point mutation (A to G, glutamic acid to glycine) from [[Part:BBa K778001|wild-type fhlA]]. | fhlA-E363G has one point mutation (A to G, glutamic acid to glycine) from [[Part:BBa K778001|wild-type fhlA]]. | ||
This mutation increased H<sub>2</sub> production under the conditions in the previous research (Viviana et.al.2009). | This mutation increased H<sub>2</sub> production under the conditions in the previous research (Viviana et.al.2009). | ||
− | Actually, in our research (team UT-Tokyo 2012[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]), this mutation increased H<sub>2</sub> production.<!--this mutation did not increase H2 production.--> | + | Actually, in our research (team UT-Tokyo 2012<!--[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]-->), this mutation increased H<sub>2</sub> production as the following figure.<!--this mutation did not increase H2 production.--> (The full construct in this experiment is [[Part:BBa K778008]]) |
− | [[Image:UT Tokyo H2 1001.png]] | + | [[Image:UT Tokyo H2 1001+eg.png]] |
+ | <!--[[Image:UT Tokyo H2 1001.png]]--> | ||
Latest revision as of 05:49, 3 October 2012
fhlA-E363G (engineered trascriptional activator fhlA with a mutation)
FhlA is the transcriptional activator of hycAp Part:BBa K778000 .E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
fhlA-E363G has one point mutation (A to G, glutamic acid to glycine) from wild-type fhlA. This mutation increased H2 production under the conditions in the previous research (Viviana et.al.2009). Actually, in our research (team UT-Tokyo 2012), this mutation increased H2 production as the following figure. (The full construct in this experiment is Part:BBa K778008)
Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli] but the results in this wiki is older than this figure above.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2096
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1375
- 1000COMPATIBLE WITH RFC[1000]