Difference between revisions of "Part:BBa K902050"
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<partinfo>BBa_K902050 short</partinfo> | <partinfo>BBa_K902050 short</partinfo> | ||
− | <html>A monooxygenase enzyme involved in the 4S desulfurization pathway. The functional DszA protein encoded by this sequence is dimeric, and is involved in the third step of the 4S biodesulfurization pathway. In this pathway, DszA carries out the conversion of DBT-sulfone to 2-hydroxybiphenyl-2-sulfinic acid through addition of an oxygen to the sulfur heteroatom. FMNH<sub>2</sub> is consumed by this reaction.<br> | + | <html>A monooxygenase enzyme involved in the 4S desulfurization pathway. The functional DszA protein encoded by this sequence is dimeric, and is involved in the third step of the 4S biodesulfurization pathway. In this pathway, DszA carries out the conversion of DBT-sulfone to 2-hydroxybiphenyl-2-sulfinic acid through addition of an oxygen to the sulfur heteroatom. FMNH<sub>2</sub> is consumed by this reaction, and therefore when this protein is employed a flavin mononucleotide oxidoreductase should also be used (such as <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K902057">BBa_K902057</a> or <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K804006">BBa_K804006</a>)<br> |
</html>[[Image:Ucalgary2012-DszApathway partspage.PNG|centre]] | </html>[[Image:Ucalgary2012-DszApathway partspage.PNG|centre]] |
Latest revision as of 02:16, 3 October 2012
DBT-sulfone monooxygenase (DszA) from R. erythropolis
A monooxygenase enzyme involved in the 4S desulfurization pathway. The functional DszA protein encoded by this sequence is dimeric, and is involved in the third step of the 4S biodesulfurization pathway. In this pathway, DszA carries out the conversion of DBT-sulfone to 2-hydroxybiphenyl-2-sulfinic acid through addition of an oxygen to the sulfur heteroatom. FMNH2 is consumed by this reaction, and therefore when this protein is employed a flavin mononucleotide oxidoreductase should also be used (such as BBa_K902057 or BBa_K804006)Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 754
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 43
Illegal NgoMIV site found at 258
Illegal NgoMIV site found at 562
Illegal NgoMIV site found at 682
Illegal AgeI site found at 217 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1066